Optical excitation and detection of neuronal activity
| العنوان: | Optical excitation and detection of neuronal activity |
|---|---|
| المؤلفون: | Viorel Nastasa, Richard Sam, Taewoo Kim, Gabriel Popescu, Parijat Sengupta, Chenfei Hu, Mingguang Shan, Minqi Wang, Martha U. Gillette |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | General Physics and Astronomy, FOS: Physical sciences, Stimulation, Optogenetics, PC12 Cells, 01 natural sciences, General Biochemistry, Genetics and Molecular Biology, Choline, 010309 optics, 03 medical and health sciences, 0103 physical sciences, High spatial resolution, Animals, Premovement neuronal activity, General Materials Science, Physics - Biological Physics, 030304 developmental biology, Neurons, Physics, 0303 health sciences, Chemistry, 010401 analytical chemistry, General Engineering, General Chemistry, Photobleaching, Molecular Imaging, Rats, 0104 chemical sciences, Electrophysiology, Phenotype, Biological Physics (physics.bio-ph), Quantitative Biology - Neurons and Cognition, Live organisms, FOS: Biological sciences, Biophysics, Neurons and Cognition (q-bio.NC), Cell activation, Intracellular transport, Excitation, Physics - Optics, Optics (physics.optics) |
| الوصف: | Optogenetics has emerged as an exciting tool for manipulating neural activity, which in turn, can modulate behavior in live organisms. However, detecting the response to the optical stimulation requires electrophysiology with physical contact or fluorescent imaging at target locations, which is often limited by photobleaching and phototoxicity. In this paper, we show that phase imaging can report the intracellular transport induced by optogenetic stimulation. We developed a multimodal instrument that can both stimulate cells with high spatial resolution and detect optical pathlength changes with nanometer scale sensitivity. We found that optical pathlength fluctuations following stimulation are consistent with active organelle transport. Furthermore, the results indicate a broadening in the transport velocity distribution, which is significantly higher in stimulated cells compared to optogenetically inactive cells. It is likely that this label-free, contactless measurement of optogenetic response will provide an enabling approach to neuroscience. Comment: 20 pages, 5 figures |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::453215c7773cc89aa30731284789da9b https://resolver.caltech.edu/CaltechAUTHORS:20181008-162019678 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....453215c7773cc89aa30731284789da9b |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |