Induction of apoptosis by Drosophila reaper, hid and grim through inhibition of IAP function
| العنوان: | Induction of apoptosis by Drosophila reaper, hid and grim through inhibition of IAP function |
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| المؤلفون: | Julie Agapite, Hermann Steller, Kimberly McCall, Lakshmi Goyal, Erika Hartwieg |
| المصدر: | The EMBO Journal. 19:589-597 |
| بيانات النشر: | Wiley, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Programmed cell death, animal structures, Mutant, Apoptosis, Genes, Insect, Eye, Models, Biological, General Biochemistry, Genetics and Molecular Biology, Inhibitor of Apoptosis Proteins, Animals, Drosophila Proteins, Molecular Biology, Gene, Alleles, Caspase, General Immunology and Microbiology, biology, Reaper, General Neuroscience, Neuropeptides, fungi, Articles, Head involution, Molecular biology, Cell biology, body regions, Phenotype, Mutation, Microscopy, Electron, Scanning, biology.protein, Insect Proteins, Drosophila, sense organs, Baculoviral IAP repeat-containing protein 3, Peptides |
| الوصف: | Induction of apoptosis in Drosophila requires the activity of three closely linked genes, reaper, hid and grim. Here we show that the proteins encoded by reaper, hid and grim activate cell death by inhibiting the anti-apoptotic activity of the Drosophila IAP1 (diap1) protein. In a genetic modifier screen, both loss-of-function and gain-of-function alleles in the endogenous diap1 gene were obtained, and the mutant proteins were functionally and biochemically characterized. Gain-of-function mutations in diap1 strongly suppressed reaper-, hid- and grim-induced apoptosis. Sequence analysis of these alleles revealed that they were caused by single amino acid changes in the baculovirus IAP repeat domains of diap1, a domain implicated in binding REAPER, HID and GRIM. Significantly, the corresponding mutant DIAP1 proteins displayed greatly reduced binding of REAPER, HID and GRIM, indicating that REAPER, HID and GRIM kill by forming a complex with DIAP1. These data provide strong in vivo evidence for a previously published model of cell death regulation in Drosophila. |
| تدمد: | 1460-2075 0261-4189 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45bb57215f48365af7b1fb47f9fb0467 https://doi.org/10.1093/emboj/19.4.589 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....45bb57215f48365af7b1fb47f9fb0467 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602075 02614189 |
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