Cyclin G2 Suppresses Estrogen-Mediated Osteogenesis through Inhibition of Wnt/β-Catenin Signaling
| العنوان: | Cyclin G2 Suppresses Estrogen-Mediated Osteogenesis through Inhibition of Wnt/β-Catenin Signaling |
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| المؤلفون: | Qi Liu, Yang Luo, Shusen Wang, Xing Liu, Shengqiang Qu, Chunyan Ji, Jinlan Gao |
| المصدر: | PLoS ONE PLoS ONE, Vol 9, Iss 3, p e89884 (2014) |
| بيانات النشر: | Public Library of Science, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | medicine.medical_specialty, Science, Cellular differentiation, Cyclin D, Osteopenia and Osteoporosis, Cyclin G2, Gene Expression, Biology, Mice, Endocrinology, Bone Density, Osteogenesis, Internal medicine, Cyclins, Molecular Cell Biology, medicine, Animals, WNT Signaling Cascade, beta Catenin, Cyclin, Multidisciplinary, Cell Death, Endocrine Physiology, Wnt signaling pathway, Osteoblast, Estrogens, Signaling Cascades, Hormones, Cell biology, RUNX2, Mice, Inbred C57BL, Wnt Proteins, medicine.anatomical_structure, biology.protein, Medicine, Women's Health, Female, C2C12, Cyclin A2, Cell Division, Research Article, Signal Transduction |
| الوصف: | Estrogen plays an important role in the maintenance of bone formation, and deficiency in the production of estrogen is directly linked to postmenopausal osteoporosis. To date, the underlying mechanisms of estrogen-mediated osteogenic differentiation are not well understood. In this study, a pluripotent mesenchymal precursor cell line C2C12 was used to induce osteogenic differentiation and subjected to detection of gene expressions or to manipulation of cyclin G2 expressions. C57BL/6 mice were used to generate bilateral ovariectomized and sham-operated mice for analysis of bone mineral density and protein expression. We identified cyclin G2, an unconventional member of cyclin, is involved in osteoblast differentiation regulated by estrogen in vivo and in vitro. In addition, the data showed that ectopic expression of cyclin G2 suppressed expression of osteoblast transcription factor Runx2 and osteogenic differentiation marker genes, as well as ALP activity and in vitro extracellular matrix mineralization. Mechanistically, Wnt/β-catenin signaling pathway is essential for cyclin G2 to inhibit osteogenic differentiation. To the best of our knowledge, the current study presents the first evidence that cyclin G2 serves as a negative regulator of both osteogenesis and Wnt/β-catenin signaling. Most importantly, the basal and 17β-estradiol-induced osteogenic differentiation was restored by overexpression of cyclin G2. These results taken together suggest that cyclin G2 may function as an endogenous suppressor of estrogen-induced osteogenic differentiation through inhibition of Wnt/β-catenin signaling. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4623fd0c6f0b4326d5eabdcc0cc5ac5e http://europepmc.org/articles/PMC3940656 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4623fd0c6f0b4326d5eabdcc0cc5ac5e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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