Distinguishing active from quiescent disease in ANCA-associated vasculitis using attenuated total reflection Fourier-transform infrared spectroscopy
| العنوان: | Distinguishing active from quiescent disease in ANCA-associated vasculitis using attenuated total reflection Fourier-transform infrared spectroscopy |
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| المؤلفون: | Daniel L. D. Freitas, Adam D. Morris, Ajay Dhaygude, Camilo L. M. Morais, Mark E. Brady, Kássio M. G. Lima, Francis Martin, Anthony W. Rowbottom |
| المصدر: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Nephrology, medicine.medical_specialty, Science, Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis, Urine, Disease, Proof of Concept Study, Gastroenterology, Article, Computational biophysics, 03 medical and health sciences, 0302 clinical medicine, Text mining, Rheumatology, Internal medicine, Spectroscopy, Fourier Transform Infrared, Humans, Medicine, Aged, 030203 arthritis & rheumatology, Multidisciplinary, business.industry, Autoantibody, Middle Aged, medicine.disease, 030104 developmental biology, Biomarker (medicine), Female, business, Vasculitis, Biomarkers |
| الوصف: | The current lack of a reliable biomarker of disease activity in anti-neutrophil cytoplasmic autoantibody (ANCA) associated vasculitis poses a significant clinical unmet need when determining relapsing or persisting disease. In this study, we demonstrate for the first time that attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy offers a novel and functional candidate biomarker, distinguishing active from quiescent disease with a high degree of accuracy. Paired blood and urine samples were collected within a single UK centre from patients with active disease, disease remission, disease controls and healthy controls. Three key biofluids were evaluated; plasma, serum and urine, with subsequent chemometric analysis and blind predictive model validation. Spectrochemical interrogation proved plasma to be the most conducive biofluid, with excellent separation between the two categories on PC2 direction (AUC 0.901) and 100% sensitivity (F-score 92.3%) for disease remission and 85.7% specificity (F-score 92.3%) for active disease on blind predictive modelling. This was independent of organ system involvement and current ANCA status, with similar findings observed on comparative analysis following successful remission-induction therapy (AUC > 0.9, 100% sensitivity for disease remission, F-score 75%). This promising technique is clinically translatable and warrants future larger study with longitudinal data, potentially aiding earlier intervention and individualisation of treatment. |
| وصف الملف: | application/pdf |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4652a9d8fb550f5b28d181cc066ff32a https://doi.org/10.1038/s41598-021-89344-8 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4652a9d8fb550f5b28d181cc066ff32a |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 20452322 |
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