Distinct contributions of the innate immune receptors TLR2 and RP105 to formation and architecture of structured lung granulomas in mice infected with Mycobacterium tuberculosis
| العنوان: | Distinct contributions of the innate immune receptors |
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| المؤلفون: | Meg L. Donovan, Helle Bielefeldt‐Ohmann, Rachel F. Rollo, Stephen J. McPherson, Thomas E. Schultz, Giorgia Mori, Jessica C. Kling, Antje Blumenthal |
| المصدر: | Immunology. 169:13-26 |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Immunology, Immunology and Allergy |
| الوصف: | Granulomas are key histopathological features of Mycobacterium tuberculosis (Mtb) infection, with complex roles in pathogen control and dissemination. Thus, understanding drivers and regulators of granuloma formation is important for improving tuberculosis diagnosis, treatment, and prevention. Yet, molecular mechanisms underpinning granuloma formation and dynamics remain poorly understood. Here we used low-dose Mtb infection of C57BL/6 mice, which elicits structured lung granulomas composed of central macrophage clusters encased by a lymphocyte mantle, alongside the disorganized lymphocyte and macrophage clusters commonly observed in Mtb-infected mice. Using gene-deficient mice, we observed that Toll-like receptor (TLR) 2 and the TLR-related Radioprotective 105 kDa protein (RP105) contributed to the extent and spatial positioning of pathology in infected lung tissues, consistent with functional cooperation between TLR2 and RP105 in the innate immune recognition of Mtb. In mice infected with the highly virulent Mtb clinical isolate HN878, TLR2, but not RP105, positively regulated the extent of central macrophage regions within structured granulomas. Moreover, RP105, but not TLR2, promoted the formation of structured lung granulomas, suggesting that the functions of RP105 as an innate immune sensor for Mtb reach beyond its roles as TLR2 co-receptor. TLR2 and RP105 contributions to lung pathology are governed by Mtb biology, as neither receptor affected the frequency or architecture of structured granulomas in mice infected with the reference strain Mtb H37Rv. Thus, by revealing distinctive as well as cooperative functions of TLR2 and RP105 in lung pathology, our data identify TLRs as molecular determinants of TB granuloma formation and architecture, and expand understanding of how interactions between innate immune receptors and Mtb shape TB disease manifestation. |
| تدمد: | 1365-2567 0019-2805 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::467705e5d4b4ed1e606eac5e43584c0c https://doi.org/10.1111/imm.13606 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....467705e5d4b4ed1e606eac5e43584c0c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652567 00192805 |
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