Amylosucrase from Neisseria polysaccharea : novel catalytic properties
| العنوان: | Amylosucrase from Neisseria polysaccharea : novel catalytic properties |
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| المؤلفون: | René-Marc Willemot, Patricia Sarçabal, Magali Remaud-Simeon, Véronique Planchot, Gabrielle Potocki de Montalk, Pierre Monsan |
| المساهمون: | Laboratoire de biochimie et technologie des glucides, Institut National de la Recherche Agronomique (INRA), ProdInra, Migration |
| المصدر: | FEBS Letters FEBS Letters, Wiley, 2000, 471 (2-3), pp.219-223 |
| بيانات النشر: | HAL CCSD, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Sucrose, Magnetic Resonance Spectroscopy, Polymers, Non-Michaelian profile, Biophysics, macromolecular substances, Fructose, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Disaccharides, Biochemistry, Catalysis, Oligosaccharide, Turanose, CATALYSE, chemistry.chemical_compound, Amylosucrase, Hydrolysis, Isomerism, Structural Biology, Genetics, Maltotriose, α-1,4-Glucan, Maltose, Molecular Biology, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, Chromatography, High Pressure Liquid, biology, Dose-Response Relationship, Drug, Cell Biology, Kinetics, Glucose, chemistry, Solubility, Glucosyltransferases, biology.protein, Glucosyltransferase, Neisseria, Trisaccharides |
| الوصف: | Amylosucrase is a glucosyltransferase that synthesises an insoluble α-glucan from sucrose. The catalytic properties of the highly purified amylosucrase from Neisseria polysaccharea were characterised. Contrary to previously published results, it was demonstrated that in the presence of sucrose alone, several reactions are catalysed, in addition to polymer synthesis: sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose synthesis – these two sucrose isomers being obtained by glucosyl transfer onto fructose. The effect of initial sucrose concentration on initial activity demonstrated a non-Michaelian profile never previously described. |
| اللغة: | English |
| تدمد: | 0014-5793 1873-3468 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::468be594233afc31bedebd1850db6d27 https://hal.inrae.fr/hal-02693033 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....468be594233afc31bedebd1850db6d27 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00145793 18733468 |
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