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Circulating cytokines present in multiple myeloma patients inhibit the osteoblastic differentiation of adipose stem cells

التفاصيل البيبلوغرافية
العنوان: Circulating cytokines present in multiple myeloma patients inhibit the osteoblastic differentiation of adipose stem cells
المؤلفون: Maurice Zaoui, Bruno Fève, Olivier Piau, Martine Auclair, Laurent Garderet, Nathalie Ferrand, Michèle Sabbah, François Delhommeau, Ladan Kobari
المساهمون: Centre de Recherche Saint-Antoine (CR Saint-Antoine), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut Universitaire de Cancérologie [Paris] (IUC), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut de Cardiométabolisme et Nutrition - Institute of Cardiometabolism and Nutrition, Centre National de Référence des Pathologies Rares de l'Insulino-Sécrétion et de l'Insulino-Sensibilité [CHu Saint-Antoine] (PRISIS), CHU Saint-Antoine [AP-HP], Service d'Hématologie clinique [CHU Pitié-Salpêtrière], CHU Pitié-Salpêtrière [AP-HP], Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Institut de Cardiométabolisme et Nutrition = Institute of Cardiometabolism and Nutrition [CHU Pitié Salpêtrière] (IHU ICAN), Sabbah, Michèle
المصدر: Leukemia
Leukemia, Nature Publishing Group: Open Access Hybrid Model Option B, 2021, Online ahead of print. ⟨10.1038/s41375-021-01428-6⟩
Leukemia, 2021, Online ahead of print. ⟨10.1038/s41375-021-01428-6⟩
سنة النشر: 2021
مصطلحات موضوعية: Cancer Research, endocrine system, animal diseases, Adipose tissue, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Downregulation and upregulation, Osteoclast, Adipocyte, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, medicine, Adipocytes, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Multiple myeloma, Cells, Cultured, 030304 developmental biology, 0303 health sciences, Osteoblasts, Stem Cells, Mesenchymal stem cell, hemic and immune systems, Cell Differentiation, Mesenchymal Stem Cells, Hematology, medicine.disease, eye diseases, Healthy Volunteers, 3. Good health, medicine.anatomical_structure, Oncology, DKK1, chemistry, 030220 oncology & carcinogenesis, Case-Control Studies, Cancer research, Cytokines, Stem cell, Multiple Myeloma, tissues
الوصف: International audience; Myeloma is characterized by bone lesions, which are related to both an increased osteoclast activity and a defect in the differentiation of medullary mesenchymal stem cells (MSCs) into osteoblasts. Outside the medullary environment, adipocytederived MSCs (ASCs) could represent a source of functional osteoblasts. However, we recently found a defect in the osteoblastic differentiation of ASCs from myeloma patients (MM-ASCs). We examined the effects of plasma from myeloma patients at diagnosis (MM-plasmas) and in complete remission (CR-plasmas) and from healthy donors on the osteoblastic differentiation of healthy donor-derived ASCs (HD-ASCs). Osteoblastogenesis in HD-ASCs was suppressed by MM-plasmas. Seven cytokines (ANG1, ENA-78, EGF, PDGF-AA/AB/BB, and TARC) were increased in MM-plasmas and separately inhibited the osteoblastic differentiation of HD-ASCs. Comparison of MM-ASCs and HD-ASCs by RNA sequencing showed that two master genes characterizing adipocyte differentiation, CD36 and PPARγ, were upregulated in MM-ASCs as compared to HD-ASCs. Finally, we demonstrated a significant increase in CD36 and PPARγ expression in HD-ASCs in the presence of MM-plasmas or the seven cytokines individually, similarly as in MM-ASCs. We conclude that specific cytokines in MM-plasmas, besides the well-known DKK1, inhibit the osteoblastic differentiation of MM-and HD-ASCs with a skewing towards adipocyte differentiation.
وصف الملف: application/pdf
تدمد: 1476-5551
0887-6924
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46d8a29d356c6084c23c3eaaf347977c
https://pubmed.ncbi.nlm.nih.gov/34556797
حقوق: EMBARGO
رقم الأكسشن: edsair.doi.dedup.....46d8a29d356c6084c23c3eaaf347977c
قاعدة البيانات: OpenAIRE
الوصف
تدمد:14765551
08876924