The immunosuppressive effect of gossypol in mice is mediated by inhibition of lymphocyte proliferation and by induction of cell apoptosis
| العنوان: | The immunosuppressive effect of gossypol in mice is mediated by inhibition of lymphocyte proliferation and by induction of cell apoptosis |
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| المؤلفون: | Li-Hui Xu, Jing-fang Di, Huanjing Shi, Xian-Hui He, Dong-Yun Ouyang, Hong-song Lu, Wen-bin Xu |
| المصدر: | Acta Pharmacologica Sinica. 30:597-604 |
| بيانات النشر: | Springer Science and Business Media LLC, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Apoptosis, Lymphocyte proliferation, Biology, Lymphocyte Activation, Mice, chemistry.chemical_compound, In vivo, Animals, Hypersensitivity, Delayed, Pharmacology (medical), Cells, Cultured, Pharmacology, Mice, Inbred BALB C, Cell growth, Gossypol, General Medicine, Molecular biology, In vitro, chemistry, Ionomycin, Immunology, Dinitrofluorobenzene, Female, Original Article, Immunosuppressive Agents, Immunostaining |
| الوصف: | To investigate the immunosuppressive effect of gossypol in mice both in vitro and in vivo. The in vitro effect of gossypol on the proliferation of lymphocytes isolated from lymph nodes of BALB/c mice was determined by CFSE staining and by an MTS assay. Lymphocyte activation and lymphoblastic transformation were evaluated with immunostaining. Cell apoptosis was detected by Annexin-V and Hoechst 33342 staining. The in vivo immunosuppressive effect of gossypol on the DTH reaction was evaluated using a mouse DTH model induced by 2,4-dinitro-1-fluorobenzene (DNFB). The thickness of the ears was measured, and the histological changes of the mouse auricles were observed after hematoxylin-eosin staining. The proliferation capacity of lymphocytes from DTH mice was also assayed. In vitro, gossypol could significantly inhibit the proliferation of mouse lymphocytes stimulated with phorbol ester plus ionomycin in a dose-dependent manner. Although the expression of the early activation antigen CD69 was not affected, the lymphoblastic transformation of both T and B lymphocyte subsets was significantly suppressed by gossypol. Moreover, gossypol could induce apoptosis of lymphocytes, and the effect was time- and dose-dependent. In vivo, the DTH reaction in mice was markedly alleviated by gossypol injected intraperitoneally. Lymphocytes from drug-treated DTH mice had a reduced proliferation capacity as compared with lymphocytes from untreated DTH mice. Gossypol treatment also markedly reduced the number of infiltrated lymphocytes in the auricles of DTH mice. Gossypol exhibited immunosuppressive effects in mice, probably by inhibition of lymphocyte proliferation and by induction of cell apoptosis. |
| تدمد: | 1745-7254 1671-4083 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47106cb1a703523fad141bae4316f811 https://doi.org/10.1038/aps.2009.35 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....47106cb1a703523fad141bae4316f811 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17457254 16714083 |
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