Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
| العنوان: | Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B |
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| المؤلفون: | Luis Alberto Scolaro, María E. Knott, Florencia N. Linero, Paula Clarisa Ellenberg, Cynthia Maeto, Viviana Castilla |
| المصدر: | Journal of General Virology. 92:2181-2190 |
| بيانات النشر: | Microbiology Society, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Sindbis virus, Heterogeneous Nuclear Ribonucleoprotein A1, viruses, Active Transport, Cell Nucleus, Virus Replication, Heterogeneous ribonucleoprotein particle, Virus, Viral Proteins, Virology, Chlorocebus aethiops, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, Animals, Immunoprecipitation, Gene silencing, Vero Cells, Junin virus, Arenavirus, biology, RNA, biology.organism_classification, Nucleoprotein, Host-Pathogen Interactions, RNA, Viral, Protein Binding |
| الوصف: | Heterogeneous nuclear ribonucleoproteins A and B (hnRNPs A/B), cellular RNA-binding proteins that participate in splicing, trafficking, translation and turnover of mRNAs, have been implicated in the life cycles of several cytoplasmic RNA viruses. Here, we demonstrate that silencing of hnRNPs A1 and A2 significantly reduces the replication of the arenavirus Junín virus (JUNV), the aetiological agent of Argentine haemorrhagic fever. While acute JUNV infection did not modify total levels of expression of hnRNPs A/B in comparison with uninfected cells, non-cytopathic persistent infection exhibited low levels of these cell proteins. Furthermore, acutely infected cells showed a cytoplasmic relocalization of overexpressed hnRNP A1, probably related to the involvement of this protein in virus replicative cycle. This cytoplasmic accumulation was also observed in cells expressing viral nucleoprotein (N), and co-immunoprecipitation studies revealed the interaction between hnRNP A1 and N protein. By contrast, a predominantly nuclear distribution of overexpressed hnRNP A1 was found during persistent infection, even in the presence of endogenous or overexpressed N protein, indicating a differential modulation of nucleo–cytoplasmic trafficking in acute and persistent JUNV infections. |
| تدمد: | 1465-2099 0022-1317 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::471873e64d383ff5298fba9e0336bb01 https://doi.org/10.1099/vir.0.030163-0 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....471873e64d383ff5298fba9e0336bb01 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14652099 00221317 |
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