Role of Adrenomedullin in Lyme Disease
| العنوان: | Role of Adrenomedullin in Lyme Disease |
|---|---|
| المؤلفون: | Janeth Yinh, Allen C. Steere, Meghan L. Marre, Shizuo Akira, Satoshi Uematsu, Linden T. Hu, Courtney T. Darcy |
| المصدر: | Infection and Immunity. 78:5307-5313 |
| بيانات النشر: | American Society for Microbiology, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Chemokine CXCL2, Interleukin-1beta, Immunology, Inflammation, Biology, Microbiology, Cell Line, Proinflammatory cytokine, Adrenomedullin, Mice, Immune system, Immunity, Osteoarthritis, Synovial Fluid, medicine, Animals, Humans, Borrelia burgdorferi, Interleukin 6, Lyme Disease, Host Response and Inflammation, Interleukin-6, Reverse Transcriptase Polymerase Chain Reaction, Tumor Necrosis Factor-alpha, Macrophages, bacterial infections and mycoses, biology.organism_classification, Mice, Inbred C57BL, Infectious Diseases, Case-Control Studies, biology.protein, Parasitology, Tumor necrosis factor alpha, medicine.symptom, hormones, hormone substitutes, and hormone antagonists |
| الوصف: | Borrelia burgdorferi stimulates a strong inflammatory response during infection of a mammalian host. To understand the mechanisms of immune regulation employed by the host to control this inflammatory response, we focused our studies on adrenomedullin, a peptide produced in response to bacterial stimuli that exhibits antimicrobial activity and regulates inflammatory responses by modulating the expression of inflammatory cytokines. Specifically, we investigated the effect of B. burgdorferi on the expression of adrenomedullin as well as the ability of adrenomedullin to dampen host inflammatory responses to the spirochete. The concentration of adrenomedullin in the synovial fluid of untreated Lyme arthritis patients was elevated compared with that in control osteoarthritis patient samples. In addition, coculture with B. burgdorferi significantly increased the expression of adrenomedullin in RAW264.7 macrophages through MyD88-, phosphatidylinositol 3-kinase (PI3-K)-, and p38-dependent signaling cascades. Furthermore, the addition of exogenous adrenomedullin to B. burgdorferi- stimulated RAW264.7 macrophages resulted in a significant decrease in the induction of proinflammatory cytokines. Taken together, these results suggest that B. burgdorferi increases the production of adrenomedullin, which in turn negatively regulates the B. burgdorferi- stimulated inflammatory response. |
| تدمد: | 1098-5522 0019-9567 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47988a3e1a077830ac32e46b183e3732 https://doi.org/10.1128/iai.00630-10 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....47988a3e1a077830ac32e46b183e3732 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985522 00199567 |
|---|