Distinguishing phosphatidic acid pools from de novo synthesis, PLD, and DGK
| العنوان: | Distinguishing phosphatidic acid pools from de novo synthesis, PLD, and DGK |
|---|---|
| المؤلفون: | Arisz, S.A., Munnik, T., Heilmann, I. |
| المساهمون: | Plant Physiology (SILS, FNWI), Green Life Sciences |
| المصدر: | Plant lipid signaling protocols, 55-62 STARTPAGE=55;ENDPAGE=62;TITLE=Plant lipid signaling protocols Methods in Molecular Biology ISBN: 9781627034005 |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | De novo synthesis, chemistry.chemical_compound, Biosynthesis, chemistry, Biochemistry, Phospholipase D, In vivo, lipids (amino acids, peptides, and proteins), Stress conditions, Phosphatidic acid, Biology, Environmental stress, Diacylglycerol kinase |
| الوصف: | In plants, phosphatidic acid (PA) functions as a metabolic precursor in the biosynthesis of glycerolipids, but it also acts as a key signaling lipid in the response to environmental stress conditions (Testerink and Munnik, J Exp Bot 62:2349-2361, 2011). In vivo (32)P-radiolabeling assays have shown the level of PA to increase within seconds/minutes of exposure to a stimulus. This response can be due to the activity of diacylglycerol kinase (DGK) and/or phospholipase D (PLD). A method is described to investigate which of the pathways is responsible for PA accumulation under a particular stress condition. First, a differential (32)P-radiolabeling protocol is used to discriminate (32)P-PA pools that are rapidly labeled versus those requiring long prelabeling times, reflecting DGK and PLD activities, respectively. Second, to specifically monitor the contribution of PLD, a transphosphatidylation assay is applied, which makes use of the artificial lipid phosphatidylbutanol as an in vivo marker of PLD activity. |
| اللغة: | English |
| ردمك: | 978-1-62703-400-5 |
| تدمد: | 1064-3745 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47e4a679cbd79055345bcc9e5ad7956b https://hdl.handle.net/11245/1.400074 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....47e4a679cbd79055345bcc9e5ad7956b |
| قاعدة البيانات: | OpenAIRE |
| ردمك: | 9781627034005 |
|---|---|
| تدمد: | 10643745 |