A Study on the Effects of IL-10 in Anti-Thy 1-Induced Glomerulonephritis in Rats
| العنوان: | A Study on the Effects of IL-10 in Anti-Thy 1-Induced Glomerulonephritis in Rats |
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| المؤلفون: | Leonie Beljaars, A. M. Van Loenen-Weemaes, Catharina Reker-Smit, Hester I. Bakker, Heni Rachmawati, Klaas Poelstra |
| المساهمون: | Groningen University Institute for Drug Exploration (GUIDE), Nanomedicine & Drug Targeting, Nanotechnology and Biophysics in Medicine (NANOBIOMED), Biopharmaceuticals, Discovery, Design and Delivery (BDDD) |
| المصدر: | University of Groningen International journal of pharmacology, 5(6), 346-353 |
| بيانات النشر: | Science Alert, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | IGA NEPHROPATHY, medicine.medical_specialty, Lipopolysaccharide, MATRIX METALLOPROTEINASES, medicine.medical_treatment, MACROPHAGE ACTIVATION, Inflammation, anti-Thy1, LIPOPOLYSACCHARIDE, Nitric oxide, chemistry.chemical_compound, Internal medicine, medicine, NEPHRITIS, TUMOR-NECROSIS-FACTOR, Pharmacology, business.industry, MESANGIAL PROLIFERATIVE GLOMERULONEPHRITIS, Glomerulonephritis, INTERLEUKIN-10, medicine.disease, Endocrinology, Cytokine, MEMBRANOUS NEPHROPATHY, chemistry, inflammation, IL-10, immunohistochemistry, CELLS, Immunohistochemistry, Mesangial proliferative glomerulonephritis, Tumor necrosis factor alpha, medicine.symptom, business, glomerulonephritis |
| الوصف: | In the present study, we examined the effects of IL-10 after 24 h in a model of acute glomerulonephritis (GN). One hour after the anti-Thy I antibody administration, a single i.v. dose of IL-10 was administered to rats. Normal rats, control nephritic rats and nephritic rats treated with IL-10, were sacrificed 24 h after administration of antibody. Samples of urine, blood and organs were subsequently collected. The effects of IL-10 were studied by quantification of various inflammatory parameters at the protein level after immunohistochemical staining and at the mRNA level by a quantitative real time PCR technique. Nitric oxide and protein content were determined in serum and in 24 h-excreted urine, respectively. The inflammatory parameters were reduced in the IL-10-treated group: in increment in glomerular CD14, ICAM-1 and MMP-13 staining induced by anti-Thy 1 injection was significantly attenuated by IL-10. In contrast, mRNA levels for CD14, IL-1 beta, TNF-alpha and MCP-1 were not different between IL-10-treated and control GN groups. In conclusion, a single i.v. dose of IL-10 suppresses the expression of several inflammatory parameters, 24 h after inducing of acute GN but at this time point mRNA levels of all parameters examined were not affected. Although its therapeutic efficacy needs further evaluation, anti-inflammatory effects of this short-lived cytokine can be found one day after its administration. |
| تدمد: | 1811-7775 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4883bf1c94ed3c47e94002f8f2350d47 https://doi.org/10.3923/ijp.2009.346.353 |
| حقوق: | RESTRICTED |
| رقم الأكسشن: | edsair.doi.dedup.....4883bf1c94ed3c47e94002f8f2350d47 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18117775 |
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