Enamel matrix derivative enhances tissue formation around scaffolds used for tissue engineering of ligaments
| العنوان: | Enamel matrix derivative enhances tissue formation around scaffolds used for tissue engineering of ligaments |
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| المؤلفون: | El Mostafa Raif, Bahaa B. Seedhom, Steven J. Brookes, Michael P. Messenger |
| المصدر: | Journal of Tissue Engineering and Regenerative Medicine. 4:96-104 |
| بيانات النشر: | Hindawi Limited, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Biomedical Engineering, Medicine (miscellaneous), chemistry.chemical_element, Calcium, Biomaterials, Extracellular matrix, Dental Enamel Proteins, Tissue engineering, Transforming Growth Factor beta, Enamel matrix derivative, Animals, Bradford protein assay, Minerals, Ligaments, Tissue Engineering, Tissue Scaffolds, Synovial Membrane, DNA, Molecular biology, In vitro, chemistry, Synovial Cell, Cattle, Electrophoresis, Polyacrylamide Gel, Amelogenin, Signal Transduction, Biomedical engineering |
| الوصف: | The following in vitro translational study investigated whether enamel matrix derivative (EMD), an approved biomimetic treatment for periodontal disease (Emdogain®) and hard-to-heal wounds (Xelma®), enhanced synovial cell colonization and protein synthesis around a scaffold used clinically for in situ tissue engineering of the torn anterior cruciate ligament (ACL). Synovial cells were enzymatically extracted from bovine synovium and dynamically seeded onto polyethylene terephthalate (PET) scaffolds. The cells were cultured in low-serum medium (0.5% FBS) for 4 weeks with either a single administration of EMD at the start of the 4 week period or multiple administrations of EMD at regular intervals throughout the 4 weeks. Samples were harvested and evaluated using the Hoechst DNA assay, BCA protein assay, cresolphthalein complexone calcium assay, SDS–PAGE, ELISA and electron microscopy. A significant increase in cell number (DNA) (p < 0.01), protein content (p < 0.01) and TGFβ1 synthesis (p < 0.01) was observed with multiple administrations of EMD. Additionally, SDS–PAGE showed an increase in high molecular weight proteins, characteristic of the fibril-forming collagens. Electron microscopy supported these findings, showing that scaffolds treated with multiple administrations of EMD were heavily coated with cells and extracellular matrix (ECM) that enveloped the fibres. Multiple administrations of EMD to synovial cell-seeded scaffolds enhanced the formation of tissue in vitro. Additionally, it was shown that EMD enhanced TGFβ1 synthesis of synovial cells, suggesting a potential mode of action for EMD's capacity to stimulate tissue regeneration. Copyright © 2009 John Wiley & Sons, Ltd. |
| تدمد: | 1932-7005 1932-6254 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::48d87719ec107303f41db7d8723e23a7 https://doi.org/10.1002/term.210 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....48d87719ec107303f41db7d8723e23a7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19327005 19326254 |
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