Platelet Activation Is Triggered by Factors Secreted by Senescent Endothelial HMEC-1 Cells In Vitro
| العنوان: | Platelet Activation Is Triggered by Factors Secreted by Senescent Endothelial HMEC-1 Cells In Vitro |
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| المؤلفون: | Daniel Mancilla, Claudio Valenzuela, Diego Méndez, Raul Segovia, Rodrigo Moore-Carrasco, Whitney Venturini, Angel Cayo, Eduardo Fuentes, Alexandra Olate-Briones, Nelson E. Brown |
| المصدر: | International Journal of Molecular Sciences Volume 21 Issue 9 International Journal of Molecular Sciences, Vol 21, Iss 3287, p 3287 (2020) |
| بيانات النشر: | MDPI, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Senescence, Blood Platelets, Cell cycle checkpoint, Cell Communication, 030204 cardiovascular system & hematology, SASP, doxorubicin, Catalysis, Article, Cell Line, Inorganic Chemistry, lcsh:Chemistry, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, In vivo, cellular senescence, Humans, Platelet, Secretion, Platelet activation, Physical and Theoretical Chemistry, Molecular Biology, lcsh:QH301-705.5, Spectroscopy, Cells, Cultured, Chemistry, Organic Chemistry, Endothelial Cells, General Medicine, Platelet Activation, In vitro, Computer Science Applications, Cell biology, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, Culture Media, Conditioned, platelets |
| الوصف: | Aging is one of the main risk factors for the development of chronic diseases, with both the vascular endothelium and platelets becoming functionally altered. Cellular senescence is a form of permanent cell cycle arrest initially described in primary cells propagated in vitro, although it can also be induced by anticancer drugs and other stressful stimuli. Attesting for the complexity of the senescent phenotype, senescent cells synthesize and secrete a wide variety of bioactive molecules. This &ldquo senescence-associated secretory phenotype&rdquo (SASP) endows senescent cells with the ability to modify the tissue microenvironment in ways that may be relevant to the development of various physiological and pathological processes. So far, however, the direct role of factors secreted by senescent endothelial cells on platelet function remains unknown. In the present work, we explore the effects of SASP factors derived from senescent endothelial cells on platelet function. To this end, we took advantage of a model in which immortalized endothelial cells (HMEC-1) were induced to senesce following exposure to doxorubicin, a chemotherapeutic drug widely used in the clinic. Our results indicate that (1) low concentrations of doxorubicin induce senescence in HMEC-1 cells (2) senescent HMEC-1 cells upregulate the expression of selected components of the SASP and (3) the media conditioned by senescent endothelial cells are capable of inducing platelet activation and aggregation. These results suggest that factors secreted by senescent endothelial cells in vivo could have a relevant role in the platelet activation observed in the elderly or in patients undergoing therapeutic stress. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::490ad8e3129d70c92a48eb1f30dcfa00 http://europepmc.org/articles/PMC7246568 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....490ad8e3129d70c92a48eb1f30dcfa00 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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