Second line molecular diagnosis for bovine tuberculosis to improve diagnostic schemes
| العنوان: | Second line molecular diagnosis for bovine tuberculosis to improve diagnostic schemes |
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| المؤلفون: | Sylvie Hénault, Maria Laura Boschiroli, Lorraine Michelet, Jean-Louis Moyen, Jennifer Tambosco, Krystel de Cruz, Claudine Karoui |
| المصدر: | PLoS ONE, Vol 13, Iss 11, p e0207614 (2018) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, lcsh:Medicine, Artificial Gene Amplification and Extension, Pathology and Laboratory Medicine, Polymerase Chain Reaction, law.invention, 0403 veterinary science, law, Medicine and Health Sciences, Rhodococcus equi, lcsh:Science, Polymerase chain reaction, Mammals, Mycobacterium bovis, Multidisciplinary, biology, Eukaryota, Nontuberculous Mycobacteria, Ruminants, DNA-Directed RNA Polymerases, 04 agricultural and veterinary sciences, Veterinary Diagnostics, Actinobacteria, Vertebrates, Tuberculosis Diagnosis and Management, Research Article, Veterinary Medicine, DNA, Bacterial, medicine.medical_specialty, 040301 veterinary sciences, 030106 microbiology, Histopathology, Research and Analysis Methods, Microbiology, Mycobacterium, 03 medical and health sciences, Bacterial Proteins, Bovines, Diagnostic Medicine, Actinomycetales, medicine, Bovine tuberculosis, Bacteriology, Animals, Molecular Biology Techniques, Molecular Biology, Retrospective Studies, Bacteria, business.industry, lcsh:R, Organisms, Biology and Life Sciences, Chaperonin 60, biology.organism_classification, Virology, Anatomical Pathology, Amniotes, Cattle, Veterinary Science, Nontuberculous mycobacteria, lcsh:Q, business, Tuberculosis, Bovine, Mycobacterium Tuberculosis |
| الوصف: | Surveillance of bovine tuberculosis (bTB) is partly based on the sanitary inspection of carcasses at the abattoir to detect bTB-like lesions which, in compliance with EU recommendations, are analysed by bacteriology and histopathology to disclose Mycobacterium bovis (or M. caprae) infection. Moreover, since 2012, a PCR method with similar sensitivity and specificity values of histopathology and bacteriology respectively is additionally employed in France, partially compensating for the weaknesses of classical diagnostic methods. We analysed a collection of bTB-like lesions from cattle presenting positive histological results albeit with negative PCR results. We present here the results of these samples, recovered from 292 animals culled between 2013 and 2016, analysed with a second line molecular diagnosis approach that consists in a combination of PCRs targeting the M. tuberculosis-M. avium complexes as well as the Mycobacterium genus and sequencing of hsp65 gene. These molecular analyses disclosed to identify the presence of non-tuberculous bacteria which could be responsible for most of these non-specific TB lesions: non tuberculous mycobacteria (24%) or Actinomycetales (56%) such as Rhodococcus equi (53%); 24% of the samples were negative. M. bovis -or any other MTBC members- was neither detected by molecular methods nor isolated in any of them at the end of the 3 months of culture. In conclusion, these results highlight the lack of specificity of histopathology and the usefulness of a first line PCR with a second line molecular diagnostic test to circumvent it. This diagnostic strategy makes it possible to reduce the number of suspect bTB cases raised at the abattoir or shortening their lock-up periods. By simplifying diagnostic schemes, the use of this tool could improve bTB surveillance and make eradication programs more efficient in the future. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::494e572ad2b99f2481dc61fff3cc0cd7 http://europepmc.org/articles/PMC6261039?pdf=render |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....494e572ad2b99f2481dc61fff3cc0cd7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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