Characterization of Two New Endo-β-1,4-xylanases from Eupenicillium parvum 4–14 and Their Applications for Production of Feruloylated Oligosaccharides
| العنوان: | Characterization of Two New Endo-β-1,4-xylanases from Eupenicillium parvum 4–14 and Their Applications for Production of Feruloylated Oligosaccharides |
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| المؤلفون: | Meijuan Xu, Shaojun Ding, Qunying Lin, Yuexin Shi, Jing Wang, Liangkun Long |
| المصدر: | Applied Biochemistry and Biotechnology. 186:816-833 |
| بيانات النشر: | Springer Science and Business Media LLC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Dietary Fiber, 0106 biological sciences, 0301 basic medicine, Avena, Oligosaccharides, Bioengineering, 01 natural sciences, Applied Microbiology and Biotechnology, Biochemistry, Pichia, Pichia pastoris, Fungal Proteins, 03 medical and health sciences, Hydrolysis, 010608 biotechnology, Eupenicillium, Glycoside hydrolase, Food science, Molecular Biology, chemistry.chemical_classification, biology, Bran, Chemistry, Substrate (chemistry), General Medicine, Hydrogen-Ion Concentration, biology.organism_classification, Xylan, Recombinant Proteins, Xylosidases, 030104 developmental biology, Enzyme, Xylans, Biotechnology |
| الوصف: | Two new endo-1,4-beta-xylanases encoding genes EpXyn1 and EpXyn3 were isolated from mesophilic fungus Eupenicillium parvum 4–14. Based on analysis of catalytic domain and phylogenetic trees, the xylanases EpXYN1 (404 aa) and EpXYN3 (220 aa) belong to glycoside hydrolase (GH) family 10 and 11, respectively. Both EpXYN1 and EpXYN3 were successfully expressed in Pichia pastoris and the recombinant enzymes were characterized using beechwood xylan, birchwood xylan, or oat spelt xylan as substrates, respectively. The optimum temperatures and pH values were 75 °C and 5.5 for EpXYN1, and 55 °C and 5.0 for EpXYN3. EpXYN1 exhibited a high stability at high temperature (65 °C) or at pH values from 8 to 10. EpXYN3 kept over 80% enzymatic activity after treatment at pH values from 3 to 10. The specific activities of EpXYN1 and EpXYN3 were 384.42 and 214.20 U/mg using beechwood xylan as substrate, respectively. EpXYN1 showed lower Km values and higher specific activities toward different xylans compared to EpXYN3. Thin-layer chromatography analysis indicated that the hydrolysis profiles of xylans or xylo-oligosacharides were different by EpXYN1and EpXYN3. EpXYN3 had a higher efficiency than EpXYN1 in production of feruloylated oligosaccharides (FOs) from de-starched wheat bran. The maximum levels of FOs released by EpXYN1 and EpXYN3 were 11.1 and 14.4 μmol/g, respectively. In conclusion, the two xylanases are potential candidates for various industrial applications. |
| تدمد: | 1559-0291 0273-2289 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4964063547210a6f1f70738a8dffd6c8 https://doi.org/10.1007/s12010-018-2775-6 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....4964063547210a6f1f70738a8dffd6c8 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15590291 02732289 |
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