Characterization of Structural Changes of Casein Micelles at Different pH Using Microscopy and Spectroscopy Techniques
| العنوان: | Characterization of Structural Changes of Casein Micelles at Different pH Using Microscopy and Spectroscopy Techniques |
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| المؤلفون: | Liliana Edith Rojas-Candelas, José Jorge Chanona-Pérez, Juan Vicente Méndez Méndez, José Antonio Morales-Hernández, Héctor Alfredo Calderón Benavides |
| المصدر: | Microscopy and Microanalysis. 28:527-536 |
| بيانات النشر: | Oxford University Press (OUP), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Instrumentation |
| الوصف: | This study aimed to evaluate the influence of pH changes on morphometric parameters of casein micelles and a general overview of their conformational structure through microscopy techniques, Raman spectroscopy and multivariate analysis. It was found that casein micelles morphology and protein secondary structure depend strongly upon pH. The changes of arithmetic average roughness (Ra), size, and shape of casein micelles at different pH are properly characterized by atomic force and cryo-transmission electron microscopy. Morphometric changes of casein micelles were correlated correctly with folding and unfolding of casein molecules as evaluated by Raman spectroscopy when the pH was varied. The novelty of this contribution consists in demonstrating that there is a close structure-functionality relationship between the morphometric parameters of proteins and their secondary structure. Knowledge about casein micelles can help improve their use of its diverse applications. |
| تدمد: | 1435-8115 1431-9276 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49b2f7b7831bb0c14e32bd856ab5d953 https://doi.org/10.1017/s1431927622000162 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....49b2f7b7831bb0c14e32bd856ab5d953 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14358115 14319276 |
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