Robust transcriptional indicators of immune cell death revealed by spatiotemporal transcriptome analyses
| العنوان: | Robust transcriptional indicators of immune cell death revealed by spatiotemporal transcriptome analyses |
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| المؤلفون: | Jose Salguero-Linares, Irene Serrano, Nerea Ruiz-Solani, Marta Salas-Gómez, Ujjal Jyoti Phukan, Victor Manuel González, Martí Bernardo-Faura, Marc Valls, David Rengel, Nuria S. Coll |
| المساهمون: | Ministerio de Ciencia, Innovación y Universidades (España), European Commission, Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), Generalitat de Catalunya, Agence Nationale de la Recherche (France), Région Midi-Pyrénées, Labex TULIP, European Research Council, University of Barcelona, Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Laboratoire des Interactions Plantes Microbes Environnement (LIPME), Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Georg-August-University = Georg-August-Universität Göttingen, Centre for Research in Agricultural Genomics (CRAG), Génome et Transcriptome - Plateforme Génomique ( GeT-PlaGe), Plateforme Génome & Transcriptome (GET), Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de pharmacologie et de biologie structurale (IPBS), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), fellowship PID2019-108595RBI00 funded by Spanish MCIN/AEI/10.13039/501100011033 (to N.S.C. and M.V.), fellowship BES-2017-080210 funded by MCIN/AEI/10.13039/501100011033 and by ``ESF Investing in your future' (to J.S.-L.), FPU19/03778 funded by MU (o Ministerio de Universidades) (to N.R.S.), by the ``Severo Ochoa Programme for Centres of Excellence in R&D' (SEV-2015-0533 and CEX2019-000902-S funded by MCIN/AEI/10.13039/501100011033), and by the CERCA Programme/Generalitat de Catalunya. Work at the LIPM was supported by the INRA SPE department (AAP2014), the Re ' gion Midi-Pyre ' ne ' es (grant 13050322), grants PID2019-108595RB-I00 funded by MCIN/AEI/10.13039/501100011033, AGL2016-78002-R funded by MCIN/AEI/10.13039/501100011033 and by ``ERDF A way of making Europe', ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010), ANR-11-IDEX-0002,UNITI,Université Fédérale de Toulouse(2011), European Project: 267196,EC:FP7:PEOPLE,FP7-PEOPLE-2010-COFUND,AGREENSKILLS(2012) |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname Molecular Plant Molecular Plant, 2022, 15 (6), pp.1059-1075. ⟨10.1016/j.molp.2022.04.010⟩ Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona |
| بيانات النشر: | Elsevier, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | hypersensitive response, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Arabidopsis thaliana, Arabidopsis, Pseudomonas syringae, Plant Science, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Effector-triggered immunity, pattern-triggered immunity, Pattern-triggered immunity, Plant immunity, effector-triggered immunity, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Plant Immunity, Molecular Biology, Plant Diseases, Cell Death, Arabidopsis Proteins, Gene Expression Profiling, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [INFO.INFO-MO]Computer Science [cs]/Modeling and Simulation, Cell death indicator, cell death indicator, Hypersensitive response, Immunitat de les plantes, Plants immunity |
| الوصف: | Recognition of a pathogen by the plant immune system often triggers a form of regulated cell death traditionally known as the hypersensitive response (HR). This type of cell death occurs precisely at the site of pathogen recognition, and it is restricted to a few cells. Extensive research has shed light on how plant immune receptors are mechanistically activated. However, two central key questions remain largely unresolved: how does cell death zonation take place, and what are the mechanisms that underpin this phenomenon? Consequently, bona fide transcriptional indicators of HR are lacking, which prevents deeper insight into its mechanisms before cell death becomes macroscopic and precludes early or live observation. In this study, to identify the transcriptional indicators of HR we used the paradigmatic Arabidopsis thaliana–Pseudomonas syringae pathosystem and performed a spatiotemporally resolved gene expression analysis that compared infected cells that will undergo HR upon pathogen recognition with bystander cells that will stay alive and activate immunity. Our data revealed unique and time-dependent differences in the repertoire of differentially expressed genes, expression profiles, and biological processes derived from tissue undergoing HR and that of its surroundings. Furthermore, we generated a pipeline based on concatenated pairwise comparisons between time, zone, and treatment that enabled us to define 13 robust transcriptional HR markers. Among these genes, the promoter of an uncharacterized AAA-ATPase was used to obtain a fluorescent reporter transgenic line that displays a strong spatiotemporally resolved signal specifically in cells that will later undergo pathogen-triggered cell death. This valuable set of genes can be used to define cells that are destined to die upon infection with HR-triggering bacteria, opening new avenues for specific and/or high-throughput techniques to study HR processes at a single-cell level. Research at CRAG was supported by grants PID2019-108595RB-I00 funded by MCIN/AEI/10.13039/501100011033 and AGL2016-78002-R funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe” (to N.S.C. and M.V.); fellowship PID2019-108595RB-I00 funded by Spanish MCIN/AEI/10.13039/501100011033 (to N.S.C. and M.V.); fellowship BES-2017-080210 funded by MCIN/AEI/10.13039/501100011033 and by “ESF Investing in your future” (to J.S.-L.); FPU19/03778 funded by MU (o Ministerio de Universidades) (to N.R.-S.); by the “Severo Ochoa Programme for Centres of Excellence in R&D” (SEV-2015-0533 and CEX2019-000902-S funded by MCIN/AEI/10.13039/501100011033); and by the CERCA Programme/Generalitat de Catalunya. Work at the LIPM was supported by the INRA SPE department (AAP2014), the Région Midi-Pyrénées (grant 13050322), and the French Laboratory of Excellence project “TULIP” (ANR-10-LABX-41; ANR-11-IDEX-0002-02). I.S. was supported by an AgreenSkills fellowship within the EU Marie-Curie FP7 COFUND People Programme (grant agreement 267196). |
| وصف الملف: | application/pdf |
| تدمد: | 1674-2052 1752-9867 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49ecea77cdbca7039830e87414e87fba http://hdl.handle.net/10261/279802 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....49ecea77cdbca7039830e87414e87fba |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16742052 17529867 |
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