Supplementary Table S1-S2; Supplementary Figures S1-S3 from Differences in Expression of Key DNA Damage Repair Genes after Epigenetic-Induced BRCAness Dictate Synthetic Lethality with PARP1 Inhibition
التفاصيل البيبلوغرافية
العنوان:
Supplementary Table S1-S2; Supplementary Figures S1-S3 from Differences in Expression of Key DNA Damage Repair Genes after Epigenetic-Induced BRCAness Dictate Synthetic Lethality with PARP1 Inhibition
American Association for Cancer Research (AACR), 2023.
سنة النشر:
2023
الوصف:
Supp. Table 1 is an IC50 calculation of each individual HDACi (SAHA, VPA, ROMI) and PARPi (ABT-888) against the four TNBC cell lines MDA-MB-231, MDA-MB-436, HCC1937(MUT) and HCC1937(RECON). Supp. Table 2 displays the combination index of the HDACi at IC20 with 2.5uM PARPi for each of the 4 cell lines described in table 1. 1 antagonism. Supp Fig 1. Is a DRGFP analysis of homologous recombination activity in to repair a GFP construct in the presence of the HDACi. We confirmed with immunofluorescence of RAD51 and marker of double strand breaks gammaH2Ax. Supp Fig 2. A) Is fluorescence microscopy displaying polyploidy in response to HDACi ROMI. B and C) Expression analysis of a set of DNA repair genes in response to HDACi (SAHA, VPA and ROMI). D) Protein expression analysis correlating with gene expression response. E) Validation of RAD51, BARD1 and FANCD2 depletion by siRNA. F-H) Cell cycle profile of MDA-MB-231 response to genetic depletion in the presence of HDACi (VPA)and PARPi (ABT-888). Supp. Fig 3. Optical and fluorescence microscopy of cells treated with combination ROMI and ABT-888.
