The use of chitosan/PLA nano-fibers by emulsion eletrospinning for periodontal tissue engineering
| العنوان: | The use of chitosan/PLA nano-fibers by emulsion eletrospinning for periodontal tissue engineering |
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| المؤلفون: | Zhanchao Ye, Enyi Zhong, Weihong Xu, Xue Yanxiang, Renze Shen, Luyuan Chen, Haicheng Ye, Yurong Yan, Jie Gao |
| المصدر: | Artificial Cells, Nanomedicine, and Biotechnology. 46:419-430 |
| بيانات النشر: | Informa UK Limited, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Periodontal tissue, Materials science, Periodontal Ligament, Polyesters, Interleukin-1beta, Nanofibers, Biomedical Engineering, Pharmaceutical Science, Medicine (miscellaneous), Biocompatible Materials, 02 engineering and technology, Chitosan, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Electricity, Polylactic acid, Osteogenesis, Cell Adhesion, Humans, RNA, Messenger, Cell Proliferation, Mechanical Phenomena, Tissue Engineering, Tissue Scaffolds, Interleukin-6, Interleukin-8, Water, Cell Differentiation, 030206 dentistry, General Medicine, 021001 nanoscience & nanotechnology, Molecular Weight, Toll-Like Receptor 4, Gene Expression Regulation, chemistry, Chemical engineering, Nanofiber, Emulsion, Emulsions, 0210 nano-technology, Hydrophobic and Hydrophilic Interactions, Biotechnology |
| الوصف: | In this study, nanofibrous scaffolds base on pure polylactic acid (PLA) and chitosan/PLA blends were fabricated by emulsion eletrospinning. By modulating their mechanical and biological properties, cell-compatible and biodegradable scaffolds were developed for periodontal bone regeneration.Pure PLA and different weight ratios of chitosan nano-particle/PLA nano-fibers were fabricated by emulsion eletrospinning. Scanning electron microscope (SEM) was performed to observe the morphology of nano-fibers. Mechanical properties of nano-fibers were tested by single fiber strength tester. Hydrophilic/hydrophobic nature of the nano-fibers was observed by stereomicroscope. In vitro degradation was also tested. Cells were seeded on nano-fibers scaffolds. Changes in cell adhesion, proliferation and osteogenic differentiation were tested by MTT assay and Alizarin Red S staining. Reverse transcription-polymerase chain reaction (RT-PCR) assay was used to evaluate the expression of (Toll-like receptor 4) TLR4, IL-6, IL-8, IL-1β, OPG, RUNX2 mRNA.It is shown that the mean diameter of nano-fibers is about 200 nm. The mean diameter of chitosan nano-particles is about 50 nm. The combination of chitosan nano-particles enhanced the mechanical properties of pure PLA nano-fibers. By adding a certain amount of chitosan nano-particles, it promoted cell adhesion. It also promoted the osteogenic differentiation of bone marrow stem cells (BMSCs) by elevating the expression of osteogenic marker genes such as BSP, Ocn, collagen I, and OPN and enhanced ECM mineralization. Nonetheless, it caused higher expression of inflammatory mediators and TLR4 of human periodontal ligament cells (hPDLCs).The combination of chitosan nano-particles enhanced the mechanical properties of pure PLA nano-fibers and increased its hydrophilicity. Pure PLA nano-fibers scaffold facilitated BMSCs proliferation. Adding an appropriate amount of chitosan nano-particles may promote its properties of cell proliferation and osteogenic differentiation. The higher expression of inflammatory mediators caused by nano-fibers may be regulated via TLR4 pathway. |
| تدمد: | 2169-141X 2169-1401 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a8560c290aa4bf7464ab4835b19bcd9 https://doi.org/10.1080/21691401.2018.1458233 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4a8560c290aa4bf7464ab4835b19bcd9 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 2169141X 21691401 |
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