Glucagon attenuates lipid accumulation in cow hepatocytes through AMPK signaling pathway activation
| العنوان: | Glucagon attenuates lipid accumulation in cow hepatocytes through AMPK signaling pathway activation |
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| المؤلفون: | Jihong Dong, Guowen Liu, Shibin Feng, Xinwei Li, Xichun Wang, Yu Li, Hongyan Ding, Sajid Ur Rahman, Zhe Wang, Jinjie Wu, Xiaobing Li |
| المصدر: | Journal of Cellular Physiology. 234:6054-6066 |
| بيانات النشر: | Wiley, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.medical_specialty, Very low-density lipoprotein, Physiology, Clinical Biochemistry, AMP-Activated Protein Kinases, Glucagon, 03 medical and health sciences, 0302 clinical medicine, Lipid oxidation, Internal medicine, medicine, Animals, Protein kinase A, Chemistry, AMPK, Lipid metabolism, Ketosis, Cell Biology, Lipid Metabolism, 030104 developmental biology, Endocrinology, 030220 oncology & carcinogenesis, Hepatocytes, Cattle, Female, lipids (amino acids, peptides, and proteins), Signal transduction, Glucagon receptor, Signal Transduction |
| الوصف: | The ketotic cows displayed hepatic lipid metabolic disorder and high blood concentration of glucagon. Importantly, adenosine monophosphate-activated protein kinase (AMPK) signaling pathway plays an important role in the hepatic lipid homeostasis. Therefore, the aim of this study was to investigate the effect of glucagon on AMPK pathway and its underlying mechanism on lipid metabolism in cow hepatocytes. Cow hepatocytes were cultured and treated with glucagon and AMPK inhibitor (BML-275). The results showed that glucagon significantly promoted the expression of glucagon receptor and increased the phosphorylation level and activity of AMPKα. Activated AMPKα increased the expression level and transcriptional activity of peroxisome proliferator-activated receptor α, which further increased the expression of fatty acid oxidation genes and lipid oxidation. Furthermore, activated AMPKα inhibited the expression level and transcriptional activity of sterol regulatory element binding protein-1c and carbohydrate response element binding protein, which decreased the expression of lipogenic genes, thereby decreasing lipid synthesis. In addition, glucagon also increased the expression of very-low-density lipoprotein (VLDL) assembly to export intracellular triglycerides (TG). Consequently, the content of intracellular TG was significantly decreased in cow hepatocytes. These results indicate that glucagon activates the AMPK signaling pathway to increase lipid oxidation and VLDL assembly and decrease lipid synthesis in cow hepatocytes, thereby reducing liver fat accumulation. |
| تدمد: | 1097-4652 0021-9541 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a882266507b153786940c68c06fe43e https://doi.org/10.1002/jcp.27258 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....4a882266507b153786940c68c06fe43e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974652 00219541 |
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