Successful Application of Ex Vivo Expanded Human Autologous Oral Mucosal Epithelium for the Treatment of Total Bilateral Limbal Stem Cell Deficiency
| العنوان: | Successful Application of Ex Vivo Expanded Human Autologous Oral Mucosal Epithelium for the Treatment of Total Bilateral Limbal Stem Cell Deficiency |
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| المؤلفون: | Sajjad Ahmad, Hardeep Singh Mudhar, Sai Kolli, Adam Meeny, Majlinda Lako, Francisco C Figueiredo |
| المصدر: | Stem Cells. 32:2135-2146 |
| بيانات النشر: | Oxford University Press (OUP), 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Pathology, medicine.medical_specialty, Cell Culture Techniques, Epithelium, Corneal, Mouth Mucosa, Cell Biology, Biology, Epithelium, Corneal Diseases, Limbal stem cell deficiency, Transplantation, medicine.anatomical_structure, Immunology, medicine, Humans, Molecular Medicine, sense organs, Mucosal epithelium, Oral mucosa, Stem cell, Ex vivo, Stem Cell Transplantation, Developmental Biology, Corneal epithelium |
| الوصف: | Ocular surface reconstruction with ex vivo expanded limbal stem cells (LSCs) is a widely used clinical treatment for patients with limbal stem cell deficiency (LSCD). This is not applicable to patients with bilateral LSCD where there are no remaining LSCs. Cultivated oral mucosa epithelium (OME) has been used as an alternative source of autologous epithelial stem cells for ocular reconstruction in few clinical trials. However, successful generation of stratified OME epithelium has only been achieved in the presence of animal feeder cells and/or animal-derived products in the culture media, likely to contribute to increased risk of pathogen transmission and graft rejection. In this study, we report generation of multilayered OME epithelium that shares many of the characteristics of corneal epithelium using a fully compliant good manufacturing practice, feeder- and animal product-free method. Proof of concept was achieved by transplantation of autologous ex vivo expanded OME in two patients with histologically confirmed bilateral total LSCD that resulted in successful reversal of LSCD in the treated eye up to 24 months. Stem Cells 2014;32:2135–2146 |
| تدمد: | 1549-4918 1066-5099 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ac9e6ca1ac57edaf9f12743c6587dd2 https://doi.org/10.1002/stem.1694 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4ac9e6ca1ac57edaf9f12743c6587dd2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15494918 10665099 |
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