Molecular alterations in the culture of lung cancer cells H1299 after exposure to high doses of ionizing radiation
| العنوان: | Molecular alterations in the culture of lung cancer cells H1299 after exposure to high doses of ionizing radiation |
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| المؤلفون: | P. G. Sakun, V. I. Voshedskiy, A. O. Sitkovskaya, I. V. Mezhevova, S. G. Vlasov, E. A. Genkova, A. A. Pushkin, T. S. Rogova |
| المصدر: | Инновационная медицина Кубани, Vol 0, Iss 3, Pp 19-23 (2021) |
| بيانات النشر: | Kubankurortresurs, OOO, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | metastatic brain lesion, cell culture, RD1-811, business.industry, complex therapy, RC86-88.9, radiosurgery, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Medical emergencies. Critical care. Intensive care. First aid, General Medicine, medicine.disease, radiation therapy, Ionizing radiation, RC666-701, Cancer research, medicine, High doses, Diseases of the circulatory (Cardiovascular) system, Surgery, Lung cancer, business, non-small cell lung cancer, RC254-282 |
| الوصف: | Objective To study the copy number of genes-components of signaling cascades involved in DNA repair, cell cycle regulation and apoptosis under the influence of high doses of ionizing radiation.Material and Мethods The study was carried out on a culture of H1299 non-small cell lung cancer cells. Cell lines were cultured in a Binder incubator (Germany) for 24 h (at 37 °C, 5% CO2 ), and then the groups were divided into therapeutic and control. The first one was irradiated with a NovalisTx, Varian linear accelerator at doses from 18 to 24 Gy, the second was not exposed to radiation. During the study, we monitored cell viability and evaluated apoptotic activity, then each sample was amplified in two iterations. During the study, cell viability was monitored, apoptotic activity was assessed, and then each sample was amplified in two replicates. The relative copy number of genetic loci was determined by Real-Time qPCR (RT-qPCR).Results When comparing the relative copy number in the genetic loci of the H1299 non-small cell lung cancer cell culture after exposure to a high dose of ionizing radiation, a statistically significant decrease in the relative copy number of the CASP3 and RBBP8 genes was found, which may indicate a decrease in the potential of caspase-mediated tumor repopulation and an increase in the radiosensitivity of tumor cells.Conclusion Exposure to high doses of ionizing radiation leads to a detrimental effect on tumor cells and allows to overcome one of the mechanisms of radioresistance – tumor cell repopulation. |
| اللغة: | Russian |
| تدمد: | 2541-9897 2500-0268 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b2e8aef204c60abaa60e2e0c95cdaab https://www.innovmedkub.ru/jour/article/view/438 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4b2e8aef204c60abaa60e2e0c95cdaab |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 25419897 25000268 |
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