Monitoring of four DNA extraction methods upstream of high-throughput sequencing of Anisakidae nematodes

التفاصيل البيبلوغرافية
العنوان: Monitoring of four DNA extraction methods upstream of high-throughput sequencing of Anisakidae nematodes
المؤلفون: Y. Seesao, C. Audebert, V. Verrez-Bagnis, S. Merlin, M. Jérôme, E. Viscogliosi, E. Dei-Cas, C.M. Aliouat-Denis, M. Gay
المصدر: Journal Of Microbiological Methods (0167-7012) (Elsevier Science Bv), 2014-07, Vol. 102, P. 69-72
بيانات النشر: Elsevier BV, 2014.
سنة النشر: 2014
مصطلحات موضوعية: Microbiology (medical), Veterinary medicine, Anisakidae, Microbiology, Anisakis, DNA sequencing, Marine fish, chemistry.chemical_compound, Ascaridoidea, Animals, Molecular Biology, Nematode, Genetics, High-throughput sequencing, biology, Pooled DNA extraction, Extraction (chemistry), Fishes, High-Throughput Nucleotide Sequencing, DNA, biology.organism_classification, DNA extraction, chemistry, Pooled dna, COX2
الوصف: Different methods were evaluated to extract DNA from pooled nematodes belonging to Anisakis, Contracaecum, Pseudoterranova and Hysterothylacium genera isolated from edible fish. Pooled DNA extraction is the first and compulsory step to allow the identification of a large number of samples through high-throughput DNA sequencing with drastic time and cost reductions.
وصف الملف: application/pdf
تدمد: 0167-7012
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b363c9224c6b2c4c0f4014de312c9fc
https://doi.org/10.1016/j.mimet.2014.05.004
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....4b363c9224c6b2c4c0f4014de312c9fc
قاعدة البيانات: OpenAIRE