Prediction and functional analysis of the replication origin of the linear plasmid pSCL2 inStreptomyces clavuligerus
| العنوان: | Prediction and functional analysis of the replication origin of the linear plasmid pSCL2 inStreptomyces clavuligerus |
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| المؤلفون: | James Piktel, Wei Wu, Shannon LeBlanc, Susan E. Jensen, Kenneth L. Roy |
| المصدر: | Canadian Journal of Microbiology. 52:293-300 |
| بيانات النشر: | Canadian Science Publishing, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | DNA Replication, DNA, Bacterial, Sequence analysis, Molecular Sequence Data, Restriction Mapping, Immunology, Streptomyces clavuligerus, Replication Origin, Polymerase Chain Reaction, Applied Microbiology and Biotechnology, Microbiology, Open Reading Frames, chemistry.chemical_compound, Plasmid, Restriction map, Bacterial Proteins, Sequence Homology, Nucleic Acid, Genetics, Molecular Biology, Base Sequence, biology, Functional analysis, Streptomycetaceae, Sequence Analysis, DNA, General Medicine, biology.organism_classification, Streptomyces, chemistry, Replicon, Actinomycetales, DNA, Plasmids |
| الوصف: | pSCL2 (120 kb), one of the linear plasmids found in Streptomyces clavuligerus NRRL3585, was isolated and partially sequenced. Computational analysis of the central region of pSCL2 revealed the presence of two open reading frames that appear to encode proteins highly homologous to RepL1 and RepL2, replication proteins from pSLA2-L, the large linear plasmid in Streptomyces rochei. The S. clavuligerus open reading frames were designated repC1 and repC2, encoding the proteins RepC1 (150 amino acids) and RepC2 (102 amino acids), respectively. The RepC and RepL proteins have identical translation features and very similar predicted secondary and tertiary structures. Functional analysis confirmed that RepC1 is essential for replication initiation of pSCL2, whereas RepC2 is dispensable but may play a role in copy number control. The RepC and RepL proteins do not show similarity to any other bacterial plasmid replication proteins. Three regions of DNA sequence, Box 1 (1050–850 bp), Box 2 (723–606 bp), and Box 3 (224–168 bp), located upstream of repC1, were also shown to be essential or very important for replication of pSCL2.Key words: pSCL2, Streptomyces clavuligerus, replication origin. |
| تدمد: | 1480-3275 0008-4166 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b4c8cbbeeb77f8c57a809e2e43e1933 https://doi.org/10.1139/w05-126 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....4b4c8cbbeeb77f8c57a809e2e43e1933 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14803275 00084166 |
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