Cancer-Associated CD43 Glycoforms as Target of Immunotherapy
العنوان: | Cancer-Associated CD43 Glycoforms as Target of Immunotherapy |
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المؤلفون: | Ricciarda Galandrini, F. Tuccillo, Iris Scala, Antonio Barbieri, P. Bonelli, Giuseppe Fiume, Giuseppe Scala, Cristina Falcone, Annamaria de Laurentiis, Angela Santoni, Ileana Quinto, Fabrizio Dal Piaz, Francesco Paolo D'Armiento, Marco Schiavone, Claudio Arra, Massimo Mallardo, Enrico Iaccino, Camillo Palmieri, Franco M. Buonaguro, Cristina Capuano, David Venzon |
المساهمون: | Tuccillo, Fm, Palmieri, C, Fiume, G, de Laurentiis, A, Schiavone, M, Falcone, C, Iaccino, E, Galandrini, R, Capuano, C, Santoni, A, D'Armiento, FRANCESCO PAOLO, Arra, C, Barbieri, A, Dal Piaz, F, Venzon, D, Bonelli, P, Buonaguro, Fm, Scala, I, Mallardo, Massimo, Quinto, I, Scala, G. |
المصدر: | Molecular Cancer Therapeutics. 13:752-762 |
بيانات النشر: | American Association for Cancer Research (AACR), 2014. |
سنة النشر: | 2014 |
مصطلحات موضوعية: | Cancer Research, Immunogen, medicine.drug_class, medicine.medical_treatment, Biology, Monoclonal antibody, Cancer Vaccines, Antibodies, Article, Epitope, Epitopes, Mice, KAPPA-B-ALPHA, FUNCTIONAL-CHARACTERIZATION, Cancer immunotherapy, Antigen, immune system diseases, hemic and lymphatic diseases, Neoplasms, Monoclonal, THYMIC ANTIGEN, Tumor Cells, Cultured, medicine, Animals, Humans, Antibodies, Monoclonal, Leukosialin, Immunotherapy, Oncology, Cultured, Mimotope, hemic and immune systems, Molecular biology, Tumor Cells, PHAGE MIMOTOPE, biology.protein, MONOCLONAL-ANTIBODIES, Antibody |
الوصف: | CD43 is a sialoglycosylated membrane protein that is involved in cell proliferation and differentiation. CD43 glycoforms that are recognized by the UN1 monoclonal antibody (mAb) were expressed in lymphoblastoid T-cell lines and solid tumors, such as breast, colon, gastric, and squamous cell lung carcinomas, while unexpressed in the normal counterparts. The cancer association of UN1/CD43 epitope suggested the possibility to use the UN1 mAb for tumor diagnosis and therapy. In this study, we show that the UN1 mAb was endowed with antitumor activity in vivo because its passive transfer inhibited the growth of UN1-positive HPB-ALL lymphoblastoid T cells in mice. Furthermore, we demonstrate that tumor inhibition was due to UN1 mAb-dependent natural killer–mediated cytotoxicity. By screening a phage-displayed random peptide library, we identified the phagotope 2/165 as a mimotope of the UN1 antigen, as it harbored a peptide sequence that was specifically recognized by the UN1 mAb and inhibited the binding of the UN1 mAb to UN1-positive tumor cells. On the basis of sequence homology with the extracellular region of CD43 (amino acids 64 to 83), the 2/165 peptide sequence was likely mimicking the protein core of the UN1/CD43 epitope. When used as vaccine in mice, the 2/165 phagotope raised antibodies against the UN1/CD43 antigen, indicating that the 2/165 phagotope mimicked the UN1 antigen structure, and could represent a novel immunogen for cancer immunotherapy. These findings support the feasibility of using monoclonal antibodies to identify cancer-associated mimotopes for immunotherapy. Mol Cancer Ther; 13(3); 752–62. ©2013 AACR . This article is highlighted in the In This Issue feature, [p. 555][1] [1]: /lookup/volpage/13/555?iss=3 |
تدمد: | 1538-8514 1535-7163 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b74d9d4a70277e6a9365033f7a97c73 https://doi.org/10.1158/1535-7163.mct-13-0651 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....4b74d9d4a70277e6a9365033f7a97c73 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 15388514 15357163 |
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