Silver staining (Campbell-Switzer) of neuronal α-synuclein assemblies induced by multiple system atrophy and Parkinson’s disease brain extracts in transgenic mice
| العنوان: | Silver staining (Campbell-Switzer) of neuronal α-synuclein assemblies induced by multiple system atrophy and Parkinson’s disease brain extracts in transgenic mice |
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| المؤلفون: | Annabelle Curry, Michel Goedert, Bernardino Ghetti, Janice L. Holton, Masami Masuda-Suzukake, Isabelle Lavenir, Daniela Passarella |
| المساهمون: | Apollo - University of Cambridge Repository |
| المصدر: | Acta Neuropathologica Communications, Vol 7, Iss 1, Pp 1-11 (2019) Acta Neuropathologica Communications |
| بيانات النشر: | BMC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Genetically modified mouse, Parkinson's disease, Transgene, animal diseases, Seeding, Mice, Transgenic, Silver staining, Transgenic, lcsh:RC346-429, Pathology and Forensic Medicine, Stereotaxic Techniques, Silver stain, Mice, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, Atrophy, mental disorders, medicine, Animals, Humans, lcsh:Neurology. Diseases of the nervous system, 030304 developmental biology, Brain Chemistry, Neurons, Synucleinopathies, α-Synuclein, 0303 health sciences, Dementia with Lewy bodies, Chemistry, Research, Neurodegeneration, Brain, Parkinson Disease, Multiple system atrophy, medicine.disease, Molecular biology, nervous system diseases, nervous system, alpha-Synuclein, Parkinson’s disease, Neurology (clinical), 030217 neurology & neurosurgery |
| الوصف: | Synucleinopathies [Parkinson’s disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy (MSA)] share filamentous α-synuclein assemblies in nerve cells and glial cells. We compared the abilities of brain extracts from MSA and PD patients to induce neuronal α-synuclein assembly and neurodegeneration following intracerebral injection in heterozygous mice transgenic for human mutant A53T α-synuclein. MSA extracts were more potent than PD extracts in inducing α-synuclein assembly and in causing neurodegeneration. MSA assemblies were Campbell-Switzer- and Gallyas-silver-positive, whereas PD assemblies were only Campbell-Switzer-positive, in confirmation of previous findings. However, induced α-synuclein inclusions were invariably Campbell-Switzer-positive and Gallyas-negative, irrespective of whether MSA or PD brain extracts were injected. The α-synuclein inclusions of non-injected homozygous mice transgenic for A53T α-synuclein were also Campbell-Switzer-positive and Gallyas-negative. These findings demonstrate that transgene expression and its intracellular environment dominated over the silver staining properties of the conformers of assembled α-synuclein. |
| وصف الملف: | text/xml; application/pdf |
| اللغة: | English |
| تدمد: | 2051-5960 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c1eefcec6c283f1641c7fafb796bf6e http://link.springer.com/article/10.1186/s40478-019-0804-5 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4c1eefcec6c283f1641c7fafb796bf6e |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 20515960 |
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