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Inhibitory effect of C-X-C motif chemokine ligand 14 on the osteogenic differentiation of human periodontal ligament cells through transforming growth factor-beta1

التفاصيل البيبلوغرافية
العنوان: Inhibitory effect of C-X-C motif chemokine ligand 14 on the osteogenic differentiation of human periodontal ligament cells through transforming growth factor-beta1
المؤلفون: Jung-Sun Moon, Sun-Hun Kim, Hyun-Mi Ko, Hae-Kyoung Shim, Jee-Hae Kang, Min-Seok Kim, Hyun-Ju Chung, Su-Young Lee
المصدر: Archives of Oral Biology. 115:104733
بيانات النشر: Elsevier BV, 2020.
سنة النشر: 2020
مصطلحات موضوعية: 0301 basic medicine, Chemokine, Periodontal Ligament, Chemokine CXCL1, medicine.medical_treatment, Transforming Growth Factor beta1, 03 medical and health sciences, 0302 clinical medicine, Western blot, Osteogenesis, medicine, Animals, Humans, Periodontal fiber, CXCL14, General Dentistry, Cells, Cultured, medicine.diagnostic_test, biology, Chemistry, Cell growth, Growth factor, Cell Differentiation, 030206 dentistry, Cell Biology, General Medicine, In vitro, Rats, Cell biology, 030104 developmental biology, Otorhinolaryngology, Transforming Growth Factors, biology.protein, Transforming growth factor
الوصف: Objective This study aimed to determine the expression of chemokine (C-X-C motif) ligand 14 (CXCL14) in pulpal and periodontal cells in vivo and in vitro, and investigate function of CXCL14 and its underlying mechanism in the proliferation and osteogenic differentiation of human periodontal ligament (hPDL) cells. Methods To determine the expression level of CXCL14 in adult rat oral tissues and in hPDL cells after application of biophysical forces, RT-PCR, western blot, and histological analyses were performed. The role of CXCL14 in proliferation and osteogenic differentiation of PDL cells was evaluated by measuring dehydrogenase activity and Alizarin red S staining. Results Strong immunoreactivity against CXCL14 was observed in the PDL tissues and pulpal cells of rat molar, and attenuated apparently by orthodontic biophysical forces. As seen in rat molar, highly expressed CXCL14 was observed in human dental pulp and hPDL cells, and attenuated obviously by biophysical tensile force. CXCL14 expression in hPDL cells was increased in incubation time-dependent manner. Proliferation of hPDL cells was inhibited dramatically by small interfering (si) RNA against CXCL14. Furthermore, dexamethasone-induced osteogenic mineralization was inhibited by recombinant human (rh) CXCL14, and augmented by CXCL14 siRNA. rhCXCL14 increased transforming growth factor-beta1 (TGF- β1) in hPDL cells. Inhibition of the cell proliferation and osteogenic differentiation of hPDL cells by CXCL14 siRNA and rhCXCL14 were restored by rhTGF-β1 and SB431542, respectively. Conclusion These results suggest that CXCL14 may play roles as a growth factor and a negative regulator of osteogenic differentiation by increasing TGF-β1 expression in hPDL cells.
تدمد: 0003-9969
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c734d685409c1538aa4e90e0ed9f953
https://doi.org/10.1016/j.archoralbio.2020.104733
حقوق: CLOSED
رقم الأكسشن: edsair.doi.dedup.....4c734d685409c1538aa4e90e0ed9f953
قاعدة البيانات: OpenAIRE
الوصف
تدمد:00039969