Possible Roles of LAMMER Kinase Lkh1 in Fission Yeast by Comparative Proteome Analysis
| العنوان: | Possible Roles of LAMMER Kinase Lkh1 in Fission Yeast by Comparative Proteome Analysis |
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| المؤلفون: | Soo-Jin Cho, Kwang-Soo Shin, Hee-Moon Park, Young Hwan Kim |
| المصدر: | Mycobiology |
| بيانات النشر: | Mycobiology (KAMJE), 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Proteome analyses, ATF1, Kinase, Lkh1, Wild type, Oxidative phosphorylation, Biology, biology.organism_classification, Microbiology, Molecular biology, Cell biology, Superoxide dismutase, Infectious Diseases, Schizosaccharomyces pombe, Proteome, biology.protein, Kinase activity, Research Article |
| الوصف: | Lkh1, the LAMMER kinase homolog in Schizosaccharomyces pombe, has dual-specific kinase activity. The lkh1+ deletion mutant exhibits flocculation upon reaching stationary phase in liquid media and filamentous adhesion growth on solid media [1]. Furthermore, the lkh1+ null mutant is sensitive to oxidative stresses, such as increased H2O2. This oxidant sensitivity is due to reduced levels of atf1+ mRNA, which reduces expression of the antioxidant enzymes catalase (Ctt1) and superoxide dismutase (SOD1) [2]. Recently, the Csx1 protein, which contains three RNA-recognition motifs, has been reported to bind directly to atf1+ mRNA in response to oxidative stress but not other stresses, and also to maintain normal levels of Atf1 by stabilizing atf1+ mRNA under oxidative-stress conditions [3]. Csx1 is phosphorylated by Lkh1 in response to oxidative stress, and the stress-activated binding of Csx1 to atf1+ mRNA is also affected by Lkh1 and Spc1 [4]. Previously, a parallel proteomic and metabolomic analysis of the Sty1p-dependent hydrogen peroxide stress response has been reported [5], but there has been no report focused on a proteome analysis of Lkh1-related proteins. The present report compares the cellular and extracellular proteomes of the wild type and lkh1+ null mutant to estimate possible roles of the Lkh1 kinase in cellular responses. |
| تدمد: | 2092-9323 1229-8093 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c83d60b1a183feb25c1f3e5ba841376 https://doi.org/10.4489/myco.2010.38.2.108 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4c83d60b1a183feb25c1f3e5ba841376 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 20929323 12298093 |
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