Protective Effects of Small-Molecule Oligopeptides Isolated from Tilapia Fish Scale on Ethanol-Induced Gastroduodenal Injury in Rats
| العنوان: | Protective Effects of Small-Molecule Oligopeptides Isolated from Tilapia Fish Scale on Ethanol-Induced Gastroduodenal Injury in Rats |
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| المؤلفون: | Xinran Liu, Jia-Wei Kang, Yong Li, Zhen Li, Na Zhu, Jia-Ni Hu, Rui Liu, Xiao-Chen Yu, Yun-Tao Hao |
| المصدر: | Nutrients Nutrients, Vol 13, Iss 2078, p 2078 (2021) Nutrients; Volume 13; Issue 6; Pages: 2078 |
| بيانات النشر: | MDPI, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, gastroduodenal injury, Antioxidant, antioxidant, medicine.medical_treatment, Interleukin-1beta, Anti-Inflammatory Agents, Apoptosis, Antioxidants, Rats, Sprague-Dawley, chemistry.chemical_compound, Pepsin, Malondialdehyde, tilapia collagen oligopeptides, TX341-641, Prostaglandin E2, Gastrin, chemistry.chemical_classification, Nutrition and Dietetics, biology, Glutathione peroxidase, Catalase, Myeloperoxidase, Cytokines, Collagen, Oligopeptides, Tilapia, medicine.drug, medicine.medical_specialty, anti-apoptosis, Article, Superoxide dismutase, 03 medical and health sciences, anti-inflammation, Internal medicine, medicine, Animals, Peroxidase, 030109 nutrition & dietetics, Ethanol, Nutrition. Foods and food supply, Superoxide Dismutase, Tumor Necrosis Factor-alpha, Rats, Gastrointestinal Tract, Disease Models, Animal, 030104 developmental biology, Endocrinology, chemistry, biology.protein, Biomarkers, Food Science |
| الوصف: | Peptic ulcer has a serious impact on people’s health around the world, and traditional medicines can cause adverse reactions. This study investigated the protective effects of tilapia collagen oligopeptides (TCOPs) on gastroduodenal injury. Seventy-two specific pathogen-free (SPF) male Sprague Dawley (SD) rats were randomly divided into six groups according to body weight: normal control group, ethanol group, whey protein group (500 mg/kg BW), and three TCOPs dose groups (250, 500, 1000 mg/kg BW). After intragastric administration for 30 days, the acute gastroduodenal injury was induced by anhydrous ethanol (5 mL/kg, intragastrically) in all groups except the normal control group. Biomarkers in gastric and duodenal tissue and serum were measured. Furthermore, western blot was used to detect the expression of apoptosis-related proteins. The results showed that the administration with TCOPs significantly reduced gastric and duodenal ulcer index, increased gastric juice pH, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities, along with the reduction of malondialdehyde (MDA) contents. TCOPs decreased tumor Necrosis Factor-α (TNF-α), interleukin-1β (IL-1β), and myeloperoxidase (MPO) levels, while interleukin– 10 (IL-10) levels were increased. Furthermore, pepsinogens 1 (PG1), pepsinogens 2 (PG2), gastrin (GAS), and the pepsinogen ratio (PGR) were decreased, the prostaglandin E2 (PGE2) and NO contents were increased after TCOPs intervention. Moreover, TCOPs up-regulated the expression of Bcl-2 and inhibited the expression of Bax and Caspase-3. In conclusion, TCOPs have protective effects on ethanol-induced gastroduodenal injury through gastrointestinal mucosal microcirculation promotion, antioxidation, anti-inflammation, and anti-apoptosis mechanisms. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2072-6643 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c8c0641e958bd503cf57c7f6c79e37d http://europepmc.org/articles/PMC8234601 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4c8c0641e958bd503cf57c7f6c79e37d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20726643 |
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