Development of sub-tropically adapted diverse provitamin-A rich maize inbreds through marker-assisted pedigree selection, their characterization and utilization in hybrid breeding
| العنوان: | Development of sub-tropically adapted diverse provitamin-A rich maize inbreds through marker-assisted pedigree selection, their characterization and utilization in hybrid breeding |
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| المؤلفون: | Saikat Pal, Rajkumar U. Zunjare, Bhukya Prakash, R. K. Khulbe, O. P. Yadav, Gulab Chand, Vignesh Muthusamy, Munegowda M Gowda, John J. Amalraj, Rashmi Chhabra, Sujay Rakshit, Bhupender Kumar, C. N. Neeraja, Jayant S. Bhat, Rajat Goswami, Firoz Hossain, Hriipulou Duo, Aanchal Baveja, Subhra J. Mishra, Mehar C. Kamboj |
| المصدر: | PLoS ONE PLoS ONE, Vol 16, Iss 2, p e0245497 (2021) |
| بيانات النشر: | Public Library of Science, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Germplasm, Pigments, Heredity, Homozygosity, Inbreeding, Vitamin A, Materials, Proa, Plant Proteins, Genetics, Multidisciplinary, Heterozygosity, Vitamin A Deficiency, Provitamins, Eukaryota, Agriculture, Plants, beta Carotene, Experimental Organism Systems, Physical Sciences, Medicine, Research Article, Breeding program, Genotype, Science, Materials Science, Crops, Biology, Research and Analysis Methods, Genes, Plant, Zea mays, Model Organisms, Plant and Algal Models, Grasses, Indel, Alleles, Hybrid, Genetic diversity, Polymorphism, Genetic, Organic Pigments, Malnutrition, Organisms, Biology and Life Sciences, Zea, Mating design, biology.organism_classification, Carotenoids, Maize, Plant Breeding, Genetic Loci, Backcrossing, Animal Studies, Edible Grain, Crop Science, Cereal Crops |
| الوصف: | Malnutrition has emerged as one of the major health problems worldwide. Traditional yellow maize has low provitamin-A (proA) content and its genetic base in proA biofortification breeding program of subtropics is extremely narrow. To diversify the proA rich germplasm, 10 elite low proA inbreds were crossed with a proA rich donor (HP702-22) having mutantcrtRB1gene. The F2populations derived from these crosses were genotyped using InDel marker specific tocrtRB1. Severe marker segregation distortion was observed. SeventeencrtRB1inbreds developed through marker-assisted pedigree breeding and seven inbreds generated using marker-assisted backcross breeding were characterized using 77 SSRs. Wide variation in gene diversity (0.08 to 0.79) and dissimilarity coefficient (0.28 to 0.84) was observed. The inbreds were grouped into three major clusters depicting the existing genetic diversity. ThecrtRB1-based inbreds possessed high β-carotene (BC: 8.72μg/g), β-cryptoxanthin (BCX: 4.58μg/g) and proA (11.01μg/g), while it was 2.35μg/g, 1.24μg/g and 2.97μg/g in checks, respectively. Based on their genetic relationships, 15 newly developedcrtRB1-based inbreds were crossed with five testers (havingcrtRB1gene) using line × tester mating design. 75 experimental hybrids withcrtRB1gene were evaluated over three locations. These experimental hybrids possessed higher BC (8.02μg/g), BCX (4.69μg/g), proA (10.37μg/g) compared to traditional hybrids used as check (BC: 2.36 μg/g, BCX: 1.53μg/g, proA: 3.13μg/g). Environment and genotypes × environment interaction had minor effects on proA content. Both additive and dominance gene action were significant for proA. The mean proportion of proA to total carotenoids (TC) was 44% amongcrtRB1-based hybrids, while 11% in traditional hybrids. BC was found to be positively correlated with BCX (r = 0.68) and proA (r = 0.98). However, no correlation was observed between proA and grain yield. Several hybrids with >10.0 t/ha grain yield with proA content >10.0 μg/g were identified. This is the first comprehensive study on development of diverse proA rich maize hybrids through marker-assisted pedigree breeding approach. The findings provides sustainable and cost-effective solution to alleviate vitamin-A deficiency. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4cad1b3f93ba0c027a79c4d316d13edc http://europepmc.org/articles/PMC7861415 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4cad1b3f93ba0c027a79c4d316d13edc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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