First evidence of transmission of an HIV-1 M/O intergroup recombinant virus
| العنوان: | First evidence of transmission of an HIV-1 M/O intergroup recombinant virus |
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| المؤلفون: | Valérie Ngono, Laure Ngono, Jean-Christophe Plantier, Thomas Mourez, Fabienne De Oliveira, Paul Alain Ngoupo, Patrice Tchendjou, Veronique B Penlap, Richard Njouom, Anfumbom Kfutwah, Serge Alain Sadeuh-Mba |
| المساهمون: | Centre Pasteur du Cameroun, Réseau International des Instituts Pasteur (RIIP), Laboratoire de virologie [Rouen], Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Département de microbiologie : Bactério, Virologie, Parasito, Hygiène-Hôpital Charles Nicolle [Rouen]-CHU Rouen, Normandie Université (NU), Groupe de Recherche sur l'Adaptation Microbienne (GRAM 2.0), Normandie Université (NU)-Normandie Université (NU)-Université de Caen Normandie (UNICAEN), Laboratoire d'Epidémiologie et de Santé Publique, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Université de Yaoundé I, We thank the Agence Nationale de Recherche sur le SIDA et les Hépatites virales (ANRS) for financial support (ANRS No12256), We thank the technical staff of Centre Pasteur of Cameroon and Rouen University Hospital. We particularly thank Marie Leoz for its helpful comments. |
| المصدر: | AIDS AIDS, Lippincott, Williams & Wilkins, 2015, 30 (1), pp.1-8. ⟨10.1097/QAD.0000000000000880⟩ |
| بيانات النشر: | HAL CCSD, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Serotype, MESH: Sequence Analysis, DNA, Genotyping Techniques, MESH: HIV-1/classification, HIV Infections, breakpoints, MESH: env Gene Products, Human Immunodeficiency Virus/genetics, Recombinant virus, Polymerase Chain Reaction, Genome, MESH: Serogroup, law.invention, law, Immunology and Allergy, Cameroon, MESH: Phylogeny, Phylogeny, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, HIV-1 M/O recombinant, MESH: Genotype, Recombination, Genetic, Genetics, Phylogenetic tree, env Gene Products, Human Immunodeficiency Virus, MESH: HIV Infections/virology, virus diseases, MESH: HIV-1/genetics, 3. Good health, Infectious Diseases, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Recombinant DNA, Female, MESH: Recombination, Genetic, MESH: Genome, Viral, Adult, Genotype, 030106 microbiology, Immunology, Genome, Viral, Biology, Serogroup, MESH: HIV-1/isolation & purification, [SDV.MP.PRO]Life Sciences [q-bio]/Microbiology and Parasitology/Protistology, 03 medical and health sciences, MESH: Genotyping Techniques, MESH: Spouses, Humans, Serotyping, Spouses, Gene, Whole genome sequencing, Genetic diversity, MESH: Humans, MESH: Serotyping, MESH: Adult, MESH: Polymerase Chain Reaction, Sequence Analysis, DNA, MESH: HIV Infections/transmission, MESH: Cameroon, Virology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Male, 030104 developmental biology, direct transmission, HIV-1, MESH: Female |
| الوصف: | Erratum in : First evidence of intra-familial transmission of an HIV-1 M/O intergroup recombinant virus: Erratum. [AIDS. 2017]; International audience; OBJECTIVE: Despite the genetic divergence between HIV-1 groups M and O, HIV-1 M/O intergroup recombinants were reported. Actually, there is no data on the transmissibility of such recombinant forms. During a surveillance of HIV genetic diversity in Cameroon, we investigated the possible direct transmission of an HIV-1 M/O recombinant virus in an HIV-infected couple.METHODS: Consecutive samples obtained from the couple were analysed for detection of dual HIV-1 groups M and O infections, and HIV-1 M/O recombinant forms. Analyses were performed using a serological and molecular algorithm based on HIV serotyping and group-specific PCRs targeting the polymerase and envelope genes. Pattern characterization of the strains found in both patients was based on complete genome sequencing. Phylogenetic and similarity profile analyses were performed to investigate the genetic relationship between viruses from both spouses and the previously described recombinant forms.RESULTS: The sero-molecular algorithm data showed a group O serotype confirmed by molecular analysis in the envelope regions, whereas molecular tests identified HIV-1 group M in the polymerase. Phylogenetic analyses and similarity profiles of the full-length genome sequences showed that both spouses were infected with a unique recombinant virus having two recombination breakpoints in the vpr gene and LTR region. No phylogenetic link was found with the previous M/O recombinants.CONCLUSION: We provide, for the first time, molecular evidence of direct transmission of an HIV-1 M/O recombinant, highlighting the potential spread of these divergent viruses. The importance of HIV-1 recombination on genetic evolution and public health when implying divergent strains as group O has to be carefully considered. |
| اللغة: | English |
| تدمد: | 0269-9370 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4cc8235fe5cde626e1a036d1a195907b https://hal-normandie-univ.archives-ouvertes.fr/hal-02442685 |
| رقم الأكسشن: | edsair.doi.dedup.....4cc8235fe5cde626e1a036d1a195907b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 02699370 |
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