Imaging of individual normal and regenerating optic fibers in the brain of living adult goldfish
| العنوان: | Imaging of individual normal and regenerating optic fibers in the brain of living adult goldfish |
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| المؤلفون: | Ronald L. Meyer, Ziren Wang, Patricia Kim, Anne M. Danks |
| المصدر: | The Journal of Comparative Neurology. 345:253-266 |
| بيانات النشر: | Wiley, 1994. |
| سنة النشر: | 1994 |
| مصطلحات موضوعية: | Superior Colliculi, Optical fiber, Nerve Crush, Biology, law.invention, chemistry.chemical_compound, Nerve Fibers, law, Goldfish, Image Processing, Computer-Assisted, Fluorescence microscope, medicine, Animals, Axon, Growth cone, Retina, General Neuroscience, fungi, Optic Nerve, Retinal, Anatomy, Carbocyanines, Nerve Regeneration, medicine.anatomical_structure, Microscopy, Fluorescence, nervous system, chemistry, Optic nerve, sense organs, Tectum |
| الوصف: | Retinal arbors in the tectum of living adult goldfish were imaged to determine whether the structural remodelling and refinement that occurs during development continues in adulthood. Individual optic fibers were labelled by making small injections of the lipophilic fluorescent dye DiI into ventral retina and viewing the exposed tectum through a fluorescence microscope equipped with a cooled CCD camera. Arbors were imaged in the living fish every 30–60 minutes for up to 7 hours. Normal adult goldfish showed no evidence of arbor remodelling during this period, though dynamic movements of varicosities present along axon segments were observed. For comparison, regenerating optic fibers were similarly imaged in fish that had undergone optic nerve crush 2–6 weeks previously. In these fish, dynamic structural changes were seen, including branch remodelling, extension and retraction of growth cones, and movement of varicosities. © 1994 Wiley-Liss, Inc. |
| تدمد: | 1096-9861 0021-9967 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4cd2a6902c6a938a5000ca10b1ffbb1d https://doi.org/10.1002/cne.903450207 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....4cd2a6902c6a938a5000ca10b1ffbb1d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10969861 00219967 |
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