Ruthenium(II) polypyridyl complexes induce BEL-7402 cell apoptosis by ROS-mediated mitochondrial pathway
| العنوان: | Ruthenium(II) polypyridyl complexes induce BEL-7402 cell apoptosis by ROS-mediated mitochondrial pathway |
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| المؤلفون: | Hong-Liang Huang, Bing-Jie Han, Jun-Hua Yao, Ji Wang, Xiang Zheng, Guang-Bin Jiang, Yun-Jun Liu, Wei Li |
| المصدر: | Journal of Inorganic Biochemistry. 141:170-179 |
| بيانات النشر: | Elsevier BV, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Pyridines, Stereochemistry, Phenazine, chemistry.chemical_element, Antineoplastic Agents, Biochemistry, Ruthenium, Inorganic Chemistry, HeLa, Structure-Activity Relationship, chemistry.chemical_compound, Coordination Complexes, Cell Line, Tumor, Humans, Cell Proliferation, chemistry.chemical_classification, Reactive oxygen species, biology, Cytotoxins, Ligand, Cell Cycle Checkpoints, DNA, Hep G2 Cells, biology.organism_classification, Intercalating Agents, Mitochondria, Gene Expression Regulation, Neoplastic, Comet assay, Proto-Oncogene Proteins c-bcl-2, chemistry, Apoptosis, Cisplatin, Reactive Oxygen Species, HeLa Cells, Plasmids |
| الوصف: | A new ligand dmdppz and its four ruthenium(II) polypyridyl complexes [Ru(dmb) 2 (dmdppz)](ClO 4 ) 2 ( 1 ), [Ru(bpy) 2 (dmdppz)](ClO 4 ) 2 ( 2 ), [Ru(phen) 2 (dmdppz)](ClO 4 ) 2 ( 3 ) and [Ru(dmp) 2 (dmdppz)](ClO 4 ) 2 ( 4 ) (where dmb, bpy, phen, dmp and dmdppz stand for 4,4′-dimethyl-2,2′-bipyridine, 2,2′-bipyridine, 1,10-phenanthroline, 2,9-dimethyl-1,10-phenanthroline and 5,8-dimethoxylpyrido[3,2- a :2′,3′- c ]phenazine, respectively) have been synthesized and characterized. Their DNA binding behaviors show that the complexes bind to calf thymus DNA by intercalation. The complexes exhibit efficient photocleavage of pBR322 DNA on irradiation. The cytotoxicity of the ligand and the complexes toward HepG-2, HeLa, MG-63, A549 and BEL-7402 were assayed by MTT ((3-(4,5-dimethylthiazo-2-yl)-2,5-diphenyltetrazolium bromide)) method. The IC 50 values of the complexes 1 , 2 , 3 and 4 toward BEL-7402 cells are 14.6, 16.8, 18.0 and 16.7 μM, respectively. Dmdppz shows no cytotoxic activity against selected cell lines. The cellular uptake, apoptosis, comet assay, reactive oxygen species (ROS), mitochondrial membrane potential and western blot analysis were investigated. These results indicate that complexes 1 – 4 exert their toxicity through the intrinsic ROS-mediated mitochondrial pathway, which is accompanied by the regulation of Bcl-2 family proteins. |
| تدمد: | 0162-0134 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4dcaa6032a000138771a5072fa8136c3 https://doi.org/10.1016/j.jinorgbio.2014.09.001 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....4dcaa6032a000138771a5072fa8136c3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 01620134 |
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