Microvolume, kinetic-dependent enzyme-linked immunosorbent assay for amoeba antibodies
| العنوان: | Microvolume, kinetic-dependent enzyme-linked immunosorbent assay for amoeba antibodies |
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| المؤلفون: | H M Mathews, K W Walls, A Y Huong |
| المصدر: | Journal of Clinical Microbiology. 19:221-224 |
| بيانات النشر: | American Society for Microbiology, 1984. |
| سنة النشر: | 1984 |
| مصطلحات موضوعية: | Microbiology (medical), chemistry.chemical_classification, Chromatography, Entamoebiasis, biology, Chemistry, Entamoeba histolytica, Enzyme-Linked Immunosorbent Assay, Amebiasis, biology.organism_classification, Molecular biology, Antibodies, Immunoglobulin G, Dilution, Absorbance, Kinetics, Enzyme, Antigen, biology.protein, Humans, Antigens, Antibody, Plate reader, Research Article |
| الوصف: | We describe a microvolume enzyme-linked immunosorbent assay based on enzyme rate kinetics. Antigens from Entamoeba histolytica were adsorbed in wells of disposable polystyrene strips containing 12 flat-bottom wells. After exposure to the serum of a patient and peroxidase-labeled anti-human immunoglobulin G, the rate of color change in specific substrate was determined by eight sequential readings of individual wells over a 2-min period with a microcomputer-controlled model MR-600 automated plate reader. The changes in absorbance readings were converted to slope values for each well by the microcomputer. Thus, 12 samples were read, and results were printed in ca. 3.5 min. Assay conditions are described and data are presented to show that this assay is quantitative for antibody and antigen concentration with a single-tube (well) dilution. |
| تدمد: | 1098-660X 0095-1137 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e020cdebd2e22df17804857192e997e https://doi.org/10.1128/jcm.19.2.221-224.1984 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4e020cdebd2e22df17804857192e997e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1098660X 00951137 |
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