Ubiquitination and deubiquitination of NP protein regulates influenza A virus RNA replication
| العنوان: | Ubiquitination and deubiquitination of NP protein regulates influenza A virus RNA replication |
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| المؤلفون: | Wen-Chi Su, Tsai-Ling Liao, Chung‐Yi Wu, King-Song Jeng, Michael M. C. Lai |
| المصدر: | The EMBO Journal |
| بيانات النشر: | Wiley, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Gene Expression Regulation, Viral, viruses, Molecular Sequence Data, RNA-dependent RNA polymerase, Genome, Viral, Biology, Virus Replication, medicine.disease_cause, Article, General Biochemistry, Genetics and Molecular Biology, Virus, Cell Line, Deubiquitinating enzyme, Viral Proteins, Downregulation and upregulation, Influenza, Human, Influenza A virus, medicine, Humans, Amino Acid Sequence, Molecular Biology, General Immunology and Microbiology, General Neuroscience, Ubiquitination, RNA-Dependent RNA Polymerase, Virology, Nucleoprotein, Nucleoproteins, Viral replication, biology.protein, RNA, Viral, Thiolester Hydrolases, Deubiquitination |
| الوصف: | Influenza A virus RNA replication requires an intricate regulatory network involving viral and cellular proteins. In this study, we examined the roles of cellular ubiquitinating/deubiquitinating enzymes (DUBs). We observed that downregulation of a cellular deubiquitinating enzyme USP11 resulted in enhanced virus production, suggesting that USP11 could inhibit influenza virus replication. Conversely, overexpression of USP11 specifically inhibited viral genomic RNA replication, and this inhibition required the deubiquitinase activity. Furthermore, we showed that USP11 interacted with PB2, PA, and NP of viral RNA replication complex, and that NP is a monoubiquitinated protein and can be deubiquitinated by USP11 in vivo. Finally, we identified K184 as the ubiquitination site on NP and this residue is crucial for virus RNA replication. We propose that ubiquitination/deubiquitination of NP can be manipulated for antiviral therapeutic purposes. |
| تدمد: | 1460-2075 0261-4189 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e4dbaa07479c63d34272e477e47315e https://doi.org/10.1038/emboj.2010.250 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4e4dbaa07479c63d34272e477e47315e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602075 02614189 |
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