A hollow-nanosphere-based microfluidic biosensor for biomonitoring of cardiac troponin I
| العنوان: | A hollow-nanosphere-based microfluidic biosensor for biomonitoring of cardiac troponin I |
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| المؤلفون: | Nawab Singh, Md. Azahar Ali, Renu John, Bansi D. Malhotra, Ashutosh Sharma, Rudra Kumar, Prabhakar Rai |
| المصدر: | Journal of Materials Chemistry B. 7:3826-3839 |
| بيانات النشر: | Royal Society of Chemistry (RSC), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Cardiac troponin, Biocompatibility, medicine.drug_class, Microfluidics, Biomedical Engineering, macromolecular substances, 02 engineering and technology, General Chemistry, General Medicine, Microfluidic biosensor, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, chemistry.chemical_compound, Myoglobin, chemistry, Troponin I, Natriuretic peptide, medicine, General Materials Science, Vanadate, 0210 nano-technology, Biomedical engineering |
| الوصف: | Cardiovascular diseases (CVDs) are the leading causes of death worldwide. Cardiac troponin I (cTnI) is a cardiac biomarker exploited extensively for early diagnosis of acute myocardial infarction (AMI). We report a highly sensitive microfluidic biosensor for the electrochemical detection of human cTnI in real patient samples. In this device, a patterned mesoporous nickel vanadate hollow-nanosphere modified chitosan (Ch-Ni3V2O8) was integrated with a microfluidic structure. Owing to its excellent redox activity and biocompatibility, larger surface, and tunable oxidation states (V5+, V4+, and V3+), the Ch-Ni3V2O8 matrix was used to functionalize the cardiac antibodies of troponin I (cAb) and amplify the electrochemical readouts. This device offered a high sensitivity of 38.88 μA ng−1 mL−1 cm−2 at a wide range of cTnI concentrations (0.005–100 ng mL−1). A low limit-of-detection of 5 pg mL−1 and high stability, high reproducibility and good selectivity were achieved due to integration of microfluidic elements with a hollow-nanosphere Ch-Ni3V2O8. This microfluidic device can be implemented to detect B-type natriuretic peptide, myoglobin, cardiac troponin C, and cardiac troponin T by functionalizing the specific antibody recognition elements and could be used for real point-of-care applications in biomedicine. |
| تدمد: | 2050-7518 2050-750X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e562f230b36644b5390d42d6cebe6df https://doi.org/10.1039/c9tb00126c |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4e562f230b36644b5390d42d6cebe6df |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20507518 2050750X |
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