Biodegradable methacrylated casein for cardiac tissue engineering applications
| العنوان: | Biodegradable methacrylated casein for cardiac tissue engineering applications |
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| المؤلفون: | M. Azam Ali, Muhammad Qasim, Maree Gould, Sonali Sudhir Sali |
| المصدر: | Journal of Materials Chemistry B. 9:1557-1567 |
| بيانات النشر: | Royal Society of Chemistry (RSC), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Surface Properties, Biomedical Engineering, Methacrylic anhydride, 02 engineering and technology, Polyvinyl alcohol, 03 medical and health sciences, chemistry.chemical_compound, Tissue engineering, Casein, Materials Testing, Humans, General Materials Science, Particle Size, Cells, Cultured, 030304 developmental biology, 0303 health sciences, Tissue Engineering, Tissue Scaffolds, Caseins, Biomaterial, Mesenchymal Stem Cells, General Chemistry, General Medicine, Biodegradation, 021001 nanoscience & nanotechnology, chemistry, Chemical engineering, Methacrylates, Surface modification, 0210 nano-technology, Infrared microscopy |
| الوصف: | Casein is a naturally derived amino group (-NH2) rich protein, that enables surface functionalization leading to hydrophilicity, which in turn facilitates better cell adhesion. Casein obtained from either commercial β-casein rich skim milk (A2 milk) or dissolved air flotation (DAF) technology was tested for its potential for tissue engineering applications in a comparative study. A novel biodegradable biomaterial was synthesized from casein by chemically modifying with methacrylic anhydride (MA) and combined with polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP) blend. The resulting methacrylated casein (CasMA) with the two polymers was processed into porous scaffolds with low and high MA concentrations to demonstrate CasMA's ease of modification and reproducibility. Fourier Transform Infrared Microscopy (FTIR) and Proton Nuclear Magnetic Resonance (1H NMR) revealed the presence of all the components and the successful modification of casein. The rheological and morphological analysis presented viscous behaviour and columnar hollow tube-like microstructures in agreement with the biomaterials' swelling and biodegradation behaviour. The live/dead in vitro assay showed high cell viability that agreed with the cell proliferation (MTT) assay in vitro, which indicated increased proliferation upon casein modification at appropriate biomaterial concentrations and volumes. This study not only showed a possible mechanism of casein methacrylation but also presented the potential use of waste materials like DAF-casein as a value-added product for tissue engineering applications. |
| تدمد: | 2050-7518 2050-750X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e5ee67b02037854f23b6ce8b9e32709 https://doi.org/10.1039/d0tb02496a |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....4e5ee67b02037854f23b6ce8b9e32709 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20507518 2050750X |
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