RAD51B plays an essential role during somatic and meiotic recombination in Physcomitrella
| العنوان: | RAD51B plays an essential role during somatic and meiotic recombination in Physcomitrella |
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| المؤلفون: | Liudmila Chelysheva, Aline Epert, Yasuko Kamisugi, Andrew C. Cuming, Sylvia Le Guin, Mathilde Grelon, Fabien Nogué, Anouchka Guyon, Florence Charlot, Didier G. Schaefer, Nathalie Vrielynck |
| المساهمون: | Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, 3Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Laboratoire de Biologie Moleculaire et Cellulaire, Institut de Biologie, Université de Neuchâtel (UNINE), Centre for Plant Sciences, Faculty of Biological Sciences, Agence National de la Recherche ANR-09-GENM-402 ANR-11-BTBR-0001-GENIUS, UK Biotechnology and Biological Research Council BB/I006710/1, INRA, Institut Jean-Pierre Bourgin, Saclay Plant Sciences UMR, Versailles, France |
| المصدر: | Nucleic Acids Research Nucleic Acids Research, Oxford University Press, 2014, 42 (19), pp.11965-11978. ⟨10.1093/nar/gku890⟩ Nucleic Acids Research 19 (42), 11965-11978. (2014) |
| بيانات النشر: | HAL CCSD, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | 0106 biological sciences, Physcomitrella, [SDV]Life Sciences [q-bio], RAD51, DNA STRAND EXCHANGE, Genome Integrity, Repair and Replication, Physcomitrella patens, 01 natural sciences, Genetic recombination, HOMOLOGOUS RECOMBINATION, XRCC2, SACCHAROMYCES-CEREVISIAE, 03 medical and health sciences, ARABIDOPSIS-THALIANA, MOSS PHYSCOMITRELLA, GENOME STABILITY, EMBRYONIC LETHALITY, NONHOMOLOGOUS DNA, REPLICATION FORK, BREAK-REPAIR, Genetics, Strand invasion, Plant Proteins, 030304 developmental biology, 0303 health sciences, biology, DNA Helicases, biology.organism_classification, Bryopsida, Meiosis, Phenotype, DMC1, Rad51 Recombinase, Homologous recombination, Gene Deletion, DNA Damage, 010606 plant biology & botany |
| الوصف: | The eukaryotic RecA homologue Rad51 is a key factor in homologous recombination and recombinational repair. Rad51-like proteins have been identified in yeast (Rad55, Rad57 and Dmc1), plants and vertebrates (RAD51B, RAD51C, RAD51D, XRCC2, XRCC3 and DMC1). RAD51 and DMC1 are the strand-exchange proteins forming a nucleofilament for strand invasion, however, the function of the paralogues in the process of homologous recombination is less clear. In yeast the two Rad51 paralogues, Rad55 and Rad57, have been shown to be involved in somatic and meiotic HR and they are essential to the formation of the Rad51/DNA nucleofilament counterbalancing the anti-recombinase activity of the SRS2 helicase. Here, we examined the role of RAD51B in the model bryophyte Physcomitrella patens. Mutant analysis shows that RAD51B is essential for the maintenance of genome integrity, for resistance to DNA damaging agents and for gene targeting. Furthermore, we set up methods to investigate meiosis in Physcomitrella and we demonstrate that the RAD51B protein is essential for meiotic homologous recombination. Finally, we show that all these functions are independent of the SRS2 anti-recombinase protein, which is in striking contrast to what is found in budding yeast where the RAD51 paralogues are fully dependent on the SRS2 anti-recombinase function. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0305-1048 1362-4962 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e9783fbab0fab8bb612e5c95cc70c9b https://hal.archives-ouvertes.fr/hal-01204143 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4e9783fbab0fab8bb612e5c95cc70c9b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 03051048 13624962 |
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