An optochemical tool for light-induced dissociation of adherens junctions to control mechanical coupling between cells
| العنوان: | An optochemical tool for light-induced dissociation of adherens junctions to control mechanical coupling between cells |
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| المؤلفون: | Tim Pflästerer, Philippe Marcq, Elisabetta Ada Cavalcanti-Adam, Adam Shellard, Roberto Mayor, Richard Wombacher, Chiara Zambarda, Dirk Ollech, Joachim P. Spatz |
| المساهمون: | Max Planck Institute for Medical Research [Heidelberg], Max-Planck-Gesellschaft, University College of London [London] (UCL), Physique et mécanique des milieux hétérogenes (UMR 7636) (PMMH), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Heidelberg University |
| المصدر: | Nature Communications Nature Communications, Nature Publishing Group, 2020, 11 (1), ⟨10.1038/s41467-020-14390-1⟩ Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020) |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Light, Optical Phenomena, Science, Xenopus, Biophysics, General Physics and Astronomy, Microscopy, Atomic Force, Traction force microscopy, General Biochemistry, Genetics and Molecular Biology, Dissociation (chemistry), Article, Cell Line, Adherens junction, 03 medical and health sciences, Xenopus laevis, 0302 clinical medicine, Antigens, CD, Cell Movement, medicine, Animals, Humans, lcsh:Science, Author Correction, [PHYS]Physics [physics], Multidisciplinary, biology, Chemistry, Cell migration, Epithelial Cells, General Chemistry, Actomyosin, Adherens Junctions, biology.organism_classification, Cadherins, Photochemical Processes, Chemical biology, Epithelium, Biomechanical Phenomena, Transmembrane domain, Cytosol, 030104 developmental biology, medicine.anatomical_structure, 030220 oncology & carcinogenesis, lcsh:Q, alpha Catenin, Biotechnology |
| الوصف: | The cadherin-catenin complex at adherens junctions (AJs) is essential for the formation of cell-cell adhesion and epithelium integrity; however, studying the dynamic regulation of AJs at high spatio-temporal resolution remains challenging. Here we present an optochemical tool which allows reconstitution of AJs by chemical dimerization of the force bearing structures and their precise light-induced dissociation. For the dimerization, we reconstitute acto-myosin connection of a tailless E-cadherin by two ways: direct recruitment of α-catenin, and linking its cytosolic tail to the transmembrane domain. Our approach enables a specific ON-OFF switch for mechanical coupling between cells that can be controlled spatially on subcellular or tissue scale via photocleavage. The combination with cell migration analysis and traction force microscopy shows a wide-range of applicability and confirms the mechanical contribution of the reconstituted AJs. Remarkably, in vivo our tool is able to control structural and functional integrity of the epidermal layer in developing Xenopus embryos. Adherens junctions (AJs) mediate cell-cell adhesion between epithelial cells but tools to study their dynamic regulation are lacking. Here the authors develop an optochemical tool to stimulate the assembly of AJs through addition of a photocleavable tool and their dissociation upon exposure to light. |
| وصف الملف: | application/pdf |
| تدمد: | 2041-1723 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ed8dc92aba66b7289fde8b71d058a92 https://pubmed.ncbi.nlm.nih.gov/32235909 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4ed8dc92aba66b7289fde8b71d058a92 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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