Transcriptome Analysis of Clavibacter Michiganensis Subsp. Michiganensis-Infected Tomatoes: A Role Of Salicylic Acid In The Host Response
| العنوان: | Transcriptome Analysis of Clavibacter Michiganensis Subsp. Michiganensis-Infected Tomatoes: A Role Of Salicylic Acid In The Host Response |
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| المؤلفون: | Sachiko Isobe, Hideki Hirakawa, Naoki Yokotani, Yoko Nishizawa, Yoshinori Hasegawa, Yoshiki Naito, Masaru Sato, Yusuke Kouzai, Eiji Yamamoto |
| المصدر: | BMC Plant Biology, Vol 21, Iss 1, Pp 1-14 (2021) BMC Plant Biology |
| بيانات النشر: | Research Square Platform LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Clavibacter michiganensis subsp. michiganensis, Plant Science, Phenylalanine ammonia-lyase, Biology, Lycopersicon, Tomato, Microbiology, Transcriptome, Plant immunity, Solanum lycopersicum, Gene expression, Gene, Plant Diseases, Gene Expression Profiling, Research, fungi, Botany, food and beverages, Salicylic acid, Clavibacter, biology.organism_classification, Plant disease, Up-Regulation, QK1-989, Host-Pathogen Interactions, Isochorismate synthase, biology.protein, RNA-seq, Clavibacter michiganensis, Cotyledon |
| الوصف: | Bacterial canker of tomato (Solanum lycopersicon) caused by the Gram-positive bacterium Clavibacter michiganensis subsp. michiganensis is an economically important disease. However, the molecular events that occur are poorly understood. To understand the host defense response to C. michiganensis infection, transcriptome sequences in tomato cotyledons were analyzed by RNA-seq. Overall, 1,877 and 540 genes were upregulated and downregulated upon infection, respectively. Gene Ontology enrichment analysis revealed that genes involved in the defense response, phosphorylation, and hormone signaling were over-represented by the infection. Induced expression of defense-associated genes suggested that the tomato response to C. michiganensis showed similarities to common plant disease responses. After infection, many resistance gene analogs (RGAs) were transcriptionally upregulated, including the expressions of some receptor-like kinases (RLKs) involved in pattern-triggered immunity. The expressions of WRKYs, NACs, HSFs, and CBP60s encoding transcription factors (TFs) were upregulated, implying their involvement in defense-associated gene expression during tomato–C. michiganensis interactions. Tomato genes orthologous to Arabidopsis EDS1, EDS5/SID1, and PAD4/EDS9, which are causal genes of salicylic acid (SA)-deficient mutants, were activated, and infection drastically stimulated SA accumulation in tomatoes. Genes involved in the phenylalanine ammonia lyase pathway were upregulated, whereas metabolic enzyme gene expression in the isochorismate synthase pathway remained unchanged. Exogenously applied SA suppressed bacterial growth and induced the expression of WRKYs, suggesting that some C. michiganensis-responsive genes are regulated by SA signaling, and SA signaling activation should improve tomato immunity against C. michiganensis. |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4effdb05e2b9e9bd2bf4c50cd8ccc6f1 https://doi.org/10.21203/rs.3.rs-593787/v1 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4effdb05e2b9e9bd2bf4c50cd8ccc6f1 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |