Cryopreservation of testicular tissue from the dog (Canis familiaris) and wild boar (Sus scrofa) by slow freezing and vitrification: Differences in cryoresistance according to cell type
| العنوان: | Cryopreservation of testicular tissue from the dog (Canis familiaris) and wild boar (Sus scrofa) by slow freezing and vitrification: Differences in cryoresistance according to cell type |
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| المؤلفون: | C.M. Picazo, C. Castaño, P. Bóveda, A. Toledano-Díaz, R. Velázquez, B. Pequeño, M.C. Esteso, J. Gadea, S. Villaverde-Morcillo, J. Cerdeira, J. Santiago-Moreno |
| المساهمون: | Agencia Estatal de Investigación (España), Comunidad de Madrid, Wild Animal Recovery Centre (CRAS), Veterinary Centre Aluche-Las Aguilas (Madrid), Castaño, C., Bóveda, P., Toledano-Díaz, A., Esteso, M C., Gadea, J., Villaverde-Morcillo, S., Santiago-Moreno, J. |
| بيانات النشر: | Elsevier, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Male, Cryopreservation, Equine, Swine, Sus scrofa, Spermatozoa, Vitrification, Sperm, Testicular tissue, Dogs, Food Animals, Semen, Freezing, Germ cells, Animals, Animal Science and Zoology, Small Animals |
| الوصف: | 8 Pág. Sperm cryopreservation is the most common procedure used to establish germplasm banks for endangered species - but sometimes sperm cells cannot be obtained. In such cases, freezing testicular tissue may be the only option. The testes contains germ cells at different stages of differentiation, including spermatogonia, primary spermatocytes, secondary spermatocytes, spermatids, and spermatozoa, among which differences in cryoresistance might be expected. The present work compares the viability and DNA integrity of 'rounded' cells, and of elongated spermatids and spermatozoa, from the dog and wild boar, following the cryopreservation of testicular tissue by slow freezing or vitrification. Cell viability was analyzed by PI/SYBR14 staining, and DNA integrity via the TUNEL technique. For wild boar, no significant differences were seen between the two methods with respect to the percentage of viable cells, nor in the percentage of cells with DNA damage. In the dog, the percentage of viable rounded germ cells (65.0 ± 2.4%) was higher (P This study was funded by grant PID2020-113288RB-100/AEI/10.13039/501100011033. |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f13282ade404701e830119f19620e05 http://hdl.handle.net/10261/304712 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4f13282ade404701e830119f19620e05 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |