Tannins from Hamamelis virginiana Bark Extract: Characterization and Improvement of the Antiviral Efficacy against Influenza A Virus and Human Papillomavirus
| العنوان: | Tannins from Hamamelis virginiana Bark Extract: Characterization and Improvement of the Antiviral Efficacy against Influenza A Virus and Human Papillomavirus |
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| المؤلفون: | Luise Florin, Aurélie Sausy, Clemens A. J. Erdelmeier, Linda L. Theisen, Fatima Boukhallouk, Gilles A. Spoden, Claude P. Muller |
| المصدر: | PLoS ONE PLoS ONE, Vol 9, Iss 1, p e88062 (2014) |
| بيانات النشر: | Public Library of Science (PLoS), 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Phytochemistry, Viral Diseases, Phytopharmacology, lcsh:Medicine, Epigallocatechin gallate, Madin Darby Canine Kidney Cells, chemistry.chemical_compound, Molecular Cell Biology, Drug Discovery, Tannin, Gallic acid, lcsh:Science, chemistry.chemical_classification, Human papillomavirus 16, Multidisciplinary, biology, Chemistry, Infectious Diseases, Biochemistry, Proanthocyanidin, Influenza A virus, visual_art, Plant Bark, visual_art.visual_art_medium, Medicine, Bark, Research Article, medicine.drug, Drugs and Devices, Human Papillomavirus Infection, Drug Research and Development, Sexually Transmitted Diseases, Hamamelis, Antiviral Agents, Dogs, Complementary and Alternative Medicine, Influenza, Human, Tannic acid, medicine, Animals, Humans, Biology, Plant Extracts, lcsh:R, Papillomavirus Infections, Hamamelis virginiana, Influenza, chemistry, biology.protein, lcsh:Q, Tannins, Neuraminidase |
| الوصف: | Antiviral activity has been demonstrated for different tannin-rich plant extracts. Since tannins of different classes and molecular weights are often found together in plant extracts and may differ in their antiviral activity, we have compared the effect against influenza A virus (IAV) of Hamamelis virginiana L. bark extract, fractions enriched in tannins of different molecular weights and individual tannins of defined structures, including pseudotannins. We demonstrate antiviral activity of the bark extract against different IAV strains, including the recently emerged H7N9, and show for the first time that a tannin-rich extract inhibits human papillomavirus (HPV) type 16 infection. As the best performing antiviral candidate, we identified a highly potent fraction against both IAV and HPV, enriched in high molecular weight condensed tannins by ultrafiltration, a simple, reproducible and easily upscalable method. This ultrafiltration concentrate and the bark extract inhibited early and, to a minor extent, later steps in the IAV life cycle and tannin-dependently inhibited HPV attachment. We observed interesting mechanistic differences between tannin structures: High molecular weight tannin containing extracts and tannic acid (1702 g/mol) inhibited both IAV receptor binding and neuraminidase activity. In contrast, low molecular weight compounds ( |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f50cd8f0b1a4a1a31989c786fa25178 https://doi.org/10.1371/journal.pone.0088062 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4f50cd8f0b1a4a1a31989c786fa25178 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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