Primary cilia have a length-dependent persistence length
| العنوان: | Primary cilia have a length-dependent persistence length |
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| المؤلفون: | Andrew Resnick, Zhe Feng, Justin Flaherty, Zhangli Peng, Yuan-Nan Young |
| المصدر: | Biomechanics and Modeling in Mechanobiology |
| بيانات النشر: | Springer Berlin Heidelberg, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Axoneme, Optical Tweezers, Paclitaxel, Models, Biological, Madin Darby Canine Kidney Cells, Mechanobiology, 03 medical and health sciences, Dogs, Primary cilia, Microtubule, Elastic Modulus, Image Processing, Computer-Assisted, Basal body, Animals, Cilia, Cytoskeleton, 030304 developmental biology, Persistence length, Physics, 0303 health sciences, Original Paper, Stochastic Processes, Mechanical Engineering, Cilium, 030302 biochemistry & molecular biology, Dynamics (mechanics), Microspheres, Elastic shell, Modeling and Simulation, Biophysics, Biotechnology |
| الوصف: | The fluctuating position of an optically trapped cilium tip under untreated and Taxol-treated conditions was used to characterize mechanical properties of the cilium axoneme and its basal body by combining experimental, analytical, and computational tools. We provide, for the first time, evidence that the persistence length of a ciliary axoneme is length-dependent; longer cilia are stiffer than shorter cilia. We demonstrate that this apparent length dependence can be understood by a combination of modeling axonemal microtubules as anisotropic elastic shells and including actomyosin-driven stochastic basal body motion. Our results also demonstrate the possibility of using observable ciliary dynamics to probe interior cytoskeletal dynamics. It is hoped that our improved characterization of cilia will result in deeper understanding of the biological function of cellular flow sensing by this organelle. |
| اللغة: | English |
| تدمد: | 1617-7940 1617-7959 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fa3a652ad92dd181403b56da2b4f12d http://europepmc.org/articles/PMC7105448 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4fa3a652ad92dd181403b56da2b4f12d |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 16177940 16177959 |
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