Emergence and transmission of New Delhi metallo‐beta‐lactamase‐5‐producing Escherichia coli Sequence Type 361 in a Tertiary Hospital in South Korea
| العنوان: | Emergence and transmission of New Delhi metallo‐beta‐lactamase‐5‐producing Escherichia coli Sequence Type 361 in a Tertiary Hospital in South Korea |
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| المؤلفون: | Qute Choi, Sun Hoe Koo, Gye Cheol Kwon, Yumi Park |
| المصدر: | Journal of Clinical Laboratory Analysis |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Microbiology (medical), Carbapenem, Clinical Biochemistry, Microbial Sensitivity Tests, Tigecycline, Biology, beta-Lactamases, Microbiology, Tertiary Care Centers, 03 medical and health sciences, Escherichia coli ST 361, 0302 clinical medicine, Drug Resistance, Multiple, Bacterial, Republic of Korea, Escherichia coli, medicine, Pulsed-field gel electrophoresis, Humans, Immunology and Allergy, New Delhi metallo‐beta‐lactamase, Escherichia coli Infections, Research Articles, Aged, Aged, 80 and over, Biochemistry (medical), Broth microdilution, Public Health, Environmental and Occupational Health, Hematology, Middle Aged, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, Electrophoresis, Gel, Pulsed-Field, Ciprofloxacin, Medical Laboratory Technology, Carbapenem-Resistant Enterobacteriaceae, 030104 developmental biology, Carbapenems, Carbapenemase‐producing Enterobacteriaceae, Amikacin, 030220 oncology & carcinogenesis, Colistin, Multilocus sequence typing, Female, Multilocus Sequence Typing, Research Article, medicine.drug |
| الوصف: | Background The emergence of carbapenem‐resistant Escherichia coli (E coli) is a serious global health threat, but little is known about carbapenemase‐producing E coli in Daejeon, South Korea. The aim of this study was to investigate characteristics of thirteen carbapenem‐resistant E coli isolates in a tertiary hospital. Methods Thirteen non‐duplicate carbapenem‐resistant E coli strains were collected from October 2017 to January 2018. Antimicrobial susceptibility was determined with the E test or disk diffusion method. The carbapenem minimum inhibitory concentrations (MICs) were determined by the agar dilution method. The colistin and tigecycline MICs were determined by broth microdilution. The resistance genes, including carbapenemase genes, were evaluated by polymerase chain reaction, and DNA sequencing was performed to characterize the genes. Pulsed‐field gel electrophoresis and multilocus sequence typing (MLST) were performed to evaluate the clonal relatedness of isolates. The clinical data of patients were retrospectively reviewed. Results All the E coli isolates harbored blaNDM‐5 gene and were resistant to most of the antimicrobial agents, such as carbapenem, cephalosporins, ciprofloxacin, and chloramphenicol, excluding amikacin and colistin. Other resistant genes, such as blaTEM‐1, blaCTX‐M‐15, blaCMY‐2, aac(6')‐Ib‐cr, and qepA, were detected. The E coli isolates harboring bla NDM‐5 belonged to ST361 (n = 11), ST12 (n = 1), ST410 (n = 1), and PFGE types A (n = 11), B (n = 1), and C (n = 1). Conclusions This study reports on an outbreak of a predominant epidemic clone, the NDM‐5 producing, multidrug‐resistant E coli ST361 isolate. These findings suggest that we should pay attention to infection control measures to limit the spread of NDM‐5‐producing pathogens. |
| تدمد: | 1098-2825 0887-8013 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fa502834629dffcec3581595585ab3b https://doi.org/10.1002/jcla.23041 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4fa502834629dffcec3581595585ab3b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10982825 08878013 |
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