Resistance of Trichoplusia ni Populations Selected by Bacillus thuringiensis Sprays to Cotton Plants Expressing Pyramided Bacillus thuringiensis Toxins Cry1Ac and Cry2Ab
| العنوان: | Resistance of Trichoplusia ni Populations Selected by Bacillus thuringiensis Sprays to Cotton Plants Expressing Pyramided Bacillus thuringiensis Toxins Cry1Ac and Cry2Ab |
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| المؤلفون: | Xiaozhao Song, Anthony M. Shelton, Alida F. Janmaat, Judith H. Myers, Ping Wang, Wendy Kain, Jian-Zhou Zhao |
| المصدر: | Applied and Environmental Microbiology. 81:1884-1890 |
| بيانات النشر: | American Society for Microbiology, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Drug Resistance, Drug resistance, Gossypium, medicine.disease_cause, Applied Microbiology and Biotechnology, Lepidoptera genitalia, Hemolysin Proteins, Bacterial Proteins, Bacillus thuringiensis, Invertebrate Microbiology, Trichoplusia, medicine, Animals, Selection, Genetic, Alleles, Bacillus thuringiensis Toxins, Ecology, biology, Toxin, fungi, Plants, Genetically Modified, biology.organism_classification, Survival Analysis, Endotoxins, Lepidoptera, Horticulture, Cry1Ac, Agronomy, Bt cotton, Food Science, Biotechnology |
| الوصف: | Two populations of Trichoplusia ni that had developed resistance to Bacillus thuringiensis sprays (Bt sprays) in commercial greenhouse vegetable production were tested for resistance to Bt cotton (BollGard II) plants expressing pyramided Cry1Ac and Cry2Ab. The T. ni colonies resistant to Bacillus thuringiensis serovar kurstaki formulations were not only resistant to the Bt toxin Cry1Ac, as previously reported, but also had a high frequency of Cry2Ab-resistant alleles, exhibiting ca. 20% survival on BollGard II foliage. BollGard II-resistant T. ni strains were established by selection with BollGard II foliage to further remove Cry2Ab-sensitive alleles in the T. ni populations. The BollGard II-resistant strains showed incomplete resistance to BollGard II, with adjusted survival values of 0.50 to 0.78 after 7 days. The resistance to the dual-toxin cotton plants was conferred by two genetically independent resistance mechanisms: one to Cry1Ac and one to Cry2Ab. The 50% lethal concentration of Cry2Ab for the resistant strain was at least 1,467-fold that for the susceptible T. ni strain. The resistance to Cry2Ab in resistant T. ni was an autosomally inherited, incompletely recessive monogenic trait. Results from this study indicate that insect populations under selection by Bt sprays in agriculture can be resistant to multiple Bt toxins and may potentially confer resistance to multitoxin Bt crops. |
| تدمد: | 1098-5336 0099-2240 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fe817621ba62fa2e368832ada547f0d https://doi.org/10.1128/aem.03382-14 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4fe817621ba62fa2e368832ada547f0d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985336 00992240 |
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