Genome-Wide Computational and Expression Analyses Reveal G-Quadruplex DNA Motifs as Conserved cis-Regulatory Elements in Human and Related Species
| العنوان: | Genome-Wide Computational and Expression Analyses Reveal G-Quadruplex DNA Motifs as Conserved cis-Regulatory Elements in Human and Related Species |
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| المؤلفون: | Vinod Kumar Yadav, Ram Krishna Thakur, Shantanu Chowdhury, Abhay Sharma, Pooja Rawal, Kangkan Halder, Rashi Halder, Anjali Verma, Farhan Mohd |
| المصدر: | Journal of Medicinal Chemistry. 51:5641-5649 |
| بيانات النشر: | American Chemical Society (ACS), 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Genetics, Genome, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, In silico, Nucleic acid sequence, Promoter, DNA, Regulatory Sequences, Nucleic Acid, G-quadruplex, chemistry.chemical_compound, chemistry, Regulatory sequence, Drug Discovery, Animals, Humans, Molecular Medicine, Promoter Regions, Genetic, Gene, HeLa Cells |
| الوصف: | Using a combination of in silico and experimental approaches, we present evidence that the G-quadruplex (G4) motif (an alternative higher-order DNA conformation) has regulatory potential. Genome-wide analyses of 99980 human, chimpanzee, mouse, and rat promoters showed enrichment of sequence with potential to adopt G4 (potential G4 or PG4) motifs near transcription start sites (TSS; P0.0001), supporting earlier findings. Interestingly, we found700 orthologously related promoters in human, mouse, and rat conserve PG4 motif(s). The corresponding genes have enriched (z score4.0) tissue-specific expression in 75 of 79 human tissues and are significantly overrepresented in signaling and regulation of cell-cycle (P10(-05)). This is supported by results from whole genome expression experiments in human HeLa S3 cells following treatment with TMPyP4 [5,10,15,20-tetra(N-methyl-4-pyridyl) porphine chloride], which is known to bind the G4 motif inside cells. Our results implicate G4-motif mediated regulation as a more general mode of transcription control than currently appreciated. |
| تدمد: | 1520-4804 0022-2623 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fed522db29071999c93935b63fff684 https://doi.org/10.1021/jm800448a |
| رقم الأكسشن: | edsair.doi.dedup.....4fed522db29071999c93935b63fff684 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15204804 00222623 |
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