Berberis microphylla G. Forst (Calafate) Berry Extract Reduces Oxidative Stress and Lipid Peroxidation of Human LDL
| العنوان: | Berberis microphylla G. Forst (Calafate) Berry Extract Reduces Oxidative Stress and Lipid Peroxidation of Human LDL |
|---|---|
| المؤلفون: | Andy J. Pérez, Claudia Radojkovic, Si Yen Chau, Daniela Nova, Lia Olivares-Caro, Claudia Mardones, José Y. Neira, Luis Bustamante |
| المصدر: | Antioxidants, Vol 9, Iss 1171, p 1171 (2020) Antioxidants Volume 9 Issue 12 |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Physiology, Linoleic acid, Clinical Biochemistry, Oxidative phosphorylation, phenolic compounds, antioxidant capacity, Berberis microphylla G. forst, medicine.disease_cause, 01 natural sciences, Biochemistry, Article, Umbilical vein, Lipid peroxidation, 03 medical and health sciences, chemistry.chemical_compound, cardiovascular disease, medicine, Food science, Molecular Biology, 030109 nutrition & dietetics, biology, 010401 analytical chemistry, lcsh:RM1-950, Berberis microphylla, human umbilical vein endothelial cells (HUVEC), Cell Biology, Ultra Liquid Chromatography with diode array detector coupled to Quadrupole-Time of Fly Mass Spectrometry (UHPLC-DAD-QTOF), biology.organism_classification, Malondialdehyde, 0104 chemical sciences, lcsh:Therapeutics. Pharmacology, chemistry, Gas chromatography–mass spectrometry, Oxidative stress |
| الوصف: | Calafate (Berberis microphylla G. Forst) is a Patagonian barberry very rich in phenolic compounds. Our aim was to demonstrate, through in vitro models, that a comprehensive characterized calafate extract has a protective role against oxidative processes associated to cardiovascular disease development. Fifty-three phenolic compounds (17 of them not previously reported in calafate), were tentatively identified by Ultra-Liquid Chromatography with Diode Array Detector, coupled to Quadrupole-Time of Fly Mass Spectrometry (UHPLC-DAD-QTOF). Fatty acids profile and metals content were studied for the first time, by Gas Chromatography Mass Spectrometry (GC-MS) and Total X-ray Fluorescence (TXRF), respectively. Linolenic and linoleic acid, and Cu, Zn, and Mn were the main relevant compounds from these groups. The bioactivity of calafate extract associated to the cardiovascular protection was evaluated using Human Umbilical Vein Endothelial Cells (HUVECs) and human low density lipoproteins (LDL) to measure oxidative stress and lipid peroxidation. The results showed that calafate extract reduced intracellular Reactive Oxygen Species (ROS) production (51%) and completely inhibited LDL oxidation and malondialdehyde (MDA) formation. These findings demonstrated the potential of the relevant mix of compounds found in calafate extract on lipoperoxidation and suggest a promising protective effect for reducing the incidence of cardiovascular disease. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2076-3921 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50322bb26b4606cc06c35b61b019c3c8 https://www.mdpi.com/2076-3921/9/12/1171 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....50322bb26b4606cc06c35b61b019c3c8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20763921 |
|---|