Antibiotic pollution in the environment caused by animal breeding has become a serious issue. The persistent release of antibiotics with animal waste may lead to antibiotic resistances in the environment, which poses a threat to human health. This study tries to provides a practical method for screening prioritized antibiotics via a comprehensive risk assessment and determination of their major sources, and put forward corresponding regulatory measures for animal industries. We investigated the occurrence and distribution of 20 antibiotics belonging to eight classes, spanning the areas of animal feed, drinking water, and animal feces on 59 animal farms in Shandong Province, China. The results showed that antibiotic contamination was prevalent in different environmental mediums (feed, feces, and drinking water) on these farms. Tetracyclines typically exhibited higher concentrations than the other classes in all samples, and the majority of antibiotics had greater concentrations in cattle feces than in pig- or chicken feces. For the antibiotic ecological risks in feces, doxycycline, tetracycline, and enrofloxacin exhibited much more toxic effects on terrestrial organisms (e.g., wheat, cucumber, and rice). Ciprofloxacin, enrofloxacin, ofloxacin, and tetracycline levels in drinking water samples can lead to high risk of antibiotic resistance, while no antibiotic posed obvious risks to human health. Based on compressive risk assessments, 11 antibiotics were prioritized to control in the animal breeding environment. Based on the survey of feeds, drinking water and animal waste from the farm, roxithromycin in the feces mainly originated from the feeds, while most prioritized antibiotics, were from extra addition in the animal breeding process (including injection and other oral routes). The key point of local antibiotic management in animal farms should be adjusted from the feed factory to the extra addition of antibiotics in animal breeding processes.
