The Subgingival Microbiome Relationship to Periodontal Disease in Older Women
| العنوان: | The Subgingival Microbiome Relationship to Periodontal Disease in Older Women |
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| المؤلفون: | Maria Tsompana, Kathy Hovey, Lu Li, Wei Zheng, Daniel I. McSkimming, Jean Wactawski-Wende, Chris Andrews, Hailey R. Banack, Robert J. Genco, Yijun Sun, Michael J. LaMonte, Michael J. Buck, V. Murugaiyan |
| المصدر: | J Dent Res |
| بيانات النشر: | SAGE Publications, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Operational taxonomic unit, Gingiva, Physiology, 03 medical and health sciences, 0302 clinical medicine, Periodontal disease, RNA, Ribosomal, 16S, Humans, Severe periodontal disease, Medicine, Microbiome, Periodontitis, General Dentistry, Aged, Aged, 80 and over, Postmenopausal women, Bacteria, business.industry, Microbiota, Research Reports, 030206 dentistry, Periodontology, Middle Aged, medicine.disease, Menopause, 030104 developmental biology, Female, Oral Microbiome, business |
| الوصف: | Understanding of the oral microbiome in relation to periodontal disease in older adults is limited. The composition and diversity of the subgingival microflora and their oligotypes in health and levels of periodontal disease were investigated in this study on older postmenopausal women. The 16S rRNA gene was sequenced using the Illumina MiSeq platform in 1,206 women aged 53 to 81 y. Presence and severity of periodontal disease were defined by Centers for Disease Control and Prevention/American Academy of Periodontology criteria. Composition of the microbiome was determined by 16S rRNA amplicon sequencing and the abundance of taxa described by the centered log2-ratio (CLR) transformed operational taxonomic unit (OTU) values. Differences according to periodontal disease status were determined by analysis of variance with Bonferroni correction. Bacteria oligotypes associated with periodontal disease and health were determined by minimum entropy decomposition and their functions estimated in silico using PICRUSt. Prevalence of none/mild, moderate, and severe periodontal disease was 25.1%, 58.3%, and 16.6%, respectively. Alpha diversity of the microbiome differed significantly across the 3 periodontal disease categories. β-Diversity differed between no/mild and severe periodontal disease, although considerable overlap was noted. Of the 267 bacterial species identified at ≥0.02% abundance, 56 (20.9%) differed significantly in abundance according to periodontal disease status. Significant linear correlations for pocket depth and clinical attachment level with bacterial amounts were observed for several taxa. Of the taxa differing in abundance according to periodontal disease status, 53% had multiple oligotypes appearing to differ between none/mild and severe periodontal disease. Among older women, taxonomic differences in subgingival microbiome composition and diversity were observed in relation to clinical periodontal disease measures. Potential differences in bacterial subspecies (oligotypes) and their function were also identified in periodontal disease compared with health. |
| تدمد: | 1544-0591 0022-0345 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50f3af39e1498f8655541e1dc587e66d https://doi.org/10.1177/0022034519860449 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....50f3af39e1498f8655541e1dc587e66d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15440591 00220345 |
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